Loop-mediated Isothermal Amplification and nested PCR of the Internal Transcribed Spacer

• Published in: PLoS neglected tropical diseases Vol. 13; no. 8
• Main Authors: Milan, Eveline Pipolo, Fernandes, José Alex Lourenço, Theodoro, Raquel Cordeiro, Zatti, Matheus da Silva, Arantes, Thales Domingos, Bay, Mônica Baumgardt, Naliato, Georggia Fatima Silva
• Format: Journal Article
• Language: English
• Published: Public Library of Science 26.08.2019
• Subjects: Anticoagulants; Care and treatment; DNA; EDTA; Genetic research; Health aspects; Histoplasmosis; Immunocompromised host; Mycoses; Pathogenic microorganisms; Polymerase chain reaction; Risk factors

Background Histoplasmosis is a neglected disease that affects mainly immunocompromised patients, presenting a progressive dissemination pattern and a high mortality rate, mainly due to delayed diagnosis, caused by slow fungal growth in culture. Therefore, a fast, suitable and cost-effective assay is...