A general method for developing immunoassays to chloracetanilide herbicides

Metolachlor, amidochlor, and butachlor were each conjugated to proteins via a thioether linkage. The molar ratios of haptens to protein ranged from 11 to 21 as determined by radioactivity. Polyclonal antibodies to metolachlor, amidochlor, and butachlor were obtained by immunizing rabbits with the co...

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Bibliographic Details
Published inJournal of agricultural and food chemistry Vol. 40; no. 2
Main Authors Feng, P.C.C. (Monsanto Agricultural Company, St. Louis, MO), Horton, S.R, Sharp, C.R
Format Journal Article
LanguageEnglish
Published 01.02.1992
Subjects
ANTICORPS
ANTICUERPOS
HERBICIDAS
HERBICIDE
METOLACHLORE
METOLACLORO
RESIDU
RESIDUOS
TECHNIQUE IMMUNOLOGIQUE
TECNICAS INMUNOLOGICAS
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Summary:Metolachlor, amidochlor, and butachlor were each conjugated to proteins via a thioether linkage. The molar ratios of haptens to protein ranged from 11 to 21 as determined by radioactivity. Polyclonal antibodies to metolachlor, amidochlor, and butachlor were obtained by immunizing rabbits with the corresponding hapten-protein conjugates. These antibodies were used in the development of sensitive inhibition ELISAs with 150 levels of less than 10 ppb. The individual ELISAs were highly specific, showing little or no cross-reactivity to other structurally similar chloroacetanilide herbicides
Bibliography:9185660
H01
ISSN:0021-8561
1520-5118