Biochemical and structural characterization of CYP124: A methyl-branched lipid ω-hydroxylase from Mycobacterium tuberculosis

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 106; no. 49; pp. 20687 - 20692
• Main Authors: Johnston, Jonathan B, Kells, Petrea M, Podust, Larissa M, Ortiz de Montellano, Paul R
• Format: Journal Article
• Language: English
• Published: National Academy of Sciences 2009
• Subjects: active sites; catalytic activity; cell walls; cytochrome P-450; hydrophobicity; immune response; immunomodulators; lipids; Mycobacterium tuberculosis; oxidation; pathogenesis; solvents; X-ray diffraction

Mycobacterium tuberculosis (Mtb) produces a variety of methyl-branched lipids that serve important functions, including modulating the immune response during pathogenesis and contributing to a robust cell wall that is impermeable to many chemical agents. Here, we report characterization of Mtb CYP12...