Qualitative correlation of the mouse neurotoxin and enzyme-linked immunoassay for deteriming Clostridium botulinum types A and B toxins

The efficacy of an enzyme-linked immunosorbent assay for botulinal toxin using polyclonal antibodies was evaluated in relation to the standard mouse assay. Qualitative tests for toxin in a meat system inoculated with Clostridium botulinum and pure cultures of various aerobic and anaerobic bacteria s...

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Published inJournal of food safety Vol. 12; no. 2; pp. 119 - 127
Main Authors Huhtanen, C.N, Whiting, R.C, Miller, A.J, Call, J.E
Format Journal Article
LanguageEnglish
Published 1992
Subjects
analytical methods
bioassays
Clostridium botulinum
enzyme-linked immunosorbent assay
food contamination
mice
neurotoxins
toxins
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Abstract The efficacy of an enzyme-linked immunosorbent assay for botulinal toxin using polyclonal antibodies was evaluated in relation to the standard mouse assay. Qualitative tests for toxin in a meat system inoculated with Clostridium botulinum and pure cultures of various aerobic and anaerobic bacteria showed that both the mouse neurotoxin and enzyme-linked immunosorbent assays detected toxin in the samples. However the ratios of mouse:ELISA activity of culture supernatant toxins of C. botulinum showed wide disparity, among strains of types A and B. Trypsin treatment resulted in a slight loss of ELISA activity but the mouse response increased.
AbstractList The efficacy of an enzyme-linked immunosorbent assay for botulinal toxin using polyclonal antibodies was evaluated in relation to the standard mouse assay. Qualitative tests for toxin in a meat system inoculated with Clostridium botulinum and pure cultures of various aerobic and anaerobic bacteria showed that both the mouse neurotoxin and enzyme-linked immunosorbent assays detected toxin in the samples. However the ratios of mouse:ELISA activity of culture supernatant toxins of C. botulinum showed wide disparity, among strains of types A and B. Trypsin treatment resulted in a slight loss of ELISA activity but the mouse response increased.
Author Miller, A.J
Whiting, R.C
Call, J.E
Huhtanen, C.N
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SubjectTerms analytical methods
bioassays
Clostridium botulinum
enzyme-linked immunosorbent assay
food contamination
mice
neurotoxins
toxins
Title Qualitative correlation of the mouse neurotoxin and enzyme-linked immunoassay for deteriming Clostridium botulinum types A and B toxins
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