Study of SLG and SCR gene for the usage as a molecular marker for self-incompatibility of oilseed rape

• Main Authors: Zaludova, J, Nix, T, Dolanska, l, Curn, V
• Format: Book
• Language: Czech
• Published: Piestany (Slovak Republic) Vyskumny ustav rastlinnej vyroby Piestany 2003
• Subjects: AUTOESTERILIDAD; AUTOSTÉRILITÉ; BRASSICA NAPUS; GENES; GÈNE; PCR; RFLP; SELF STERILITY
• ISBN: 9788088790297; 8088790298

Oil seed rape (Brassica napus L.) cultivars, donors of quality (SC) and self-incompatible (SI) lines have been analysed using identification of S-locus. In several Brassica napus cultivars one S-locus SLG gene was detected as dominant and the second S-locus as recessive. Amplification class II SLG gene screened recessive gene in all analysed samples (SC and SI). S-haplotypes were analysed by PCR-RFLP. Brassica napus cultivars had mostly an identical electrophoretic profile conforming nonfunctional A10 (dominant) allele originating form B. campestris genome. In B. napus A10 allele is localised in genome A. The presence of A10 allele always corresponded to SC phenotype. The functional recessive SLG gene is probably localised in genome C. Recently was identified SCR gene (S-locus Cys-rich) and it is supposed to be a male determinant of SI. This observation has been shown in Brassica oleracea and Brassca rapa only. We report here the identification and sequency analysis of SCR gene from class II S-haplotypes of Brassica napus.