Effect of exogenous monosaccharide and lectins on Hill reaction activity in isolated chloroplasts of intact and infected plants

• Published in: Бюллетень Главного ботанического сада (Российская Федерация) Vol. 193; pp. 158 - 169
• Main Author: Babosha, A.V., Russian Academy of Sciences, Moscow (Russian Federation). The N.V. Tsitsin Central Botanical Garden
• Format: Journal Article
• Language: Russian
• Published: 2007
• Subjects: AGENT PATHOGENE; CELLS; CELLULE; CELULAS; CHLOROPLASTE; CHLOROPLASTS; CLOROPLASTO; ERYSIPHE GRAMINIS; FEDERACION DE RUSIA; FEDERATION DE RUSSIE; FOTOSINTESIS; FUSARIUM; http://www.fao.org/aos/agrovoc#c_12644; http://www.fao.org/aos/agrovoc#c_1418; http://www.fao.org/aos/agrovoc#c_24042; http://www.fao.org/aos/agrovoc#c_27196; http://www.fao.org/aos/agrovoc#c_3156; http://www.fao.org/aos/agrovoc#c_33240; http://www.fao.org/aos/agrovoc#c_3852; http://www.fao.org/aos/agrovoc#c_4769; http://www.fao.org/aos/agrovoc#c_5630; http://www.fao.org/aos/agrovoc#c_5651; http://www.fao.org/aos/agrovoc#c_5691; http://www.fao.org/aos/agrovoc#c_5812; http://www.fao.org/aos/agrovoc#c_7951; http://www.fao.org/aos/agrovoc#c_9824; INFECCION; INFECTION; LECTINAS; LECTINE; LECTINS; METABOLISM; METABOLISME; METABOLISMO; MONOSACARIDOS; MONOSACCHARIDE; MONOSACCHARIDES; ORGANISMOS PATOGENOS; PATHOGENS; PECTINAS; PECTINE; PECTINS; PEPTIDE; PEPTIDES; PEPTIDOS; PHOTOSYNTHESE; PHOTOSYNTHESIS; RUSSIAN FEDERATION; TRITICUM AESTIVUM
• ISSN: 0366-502X

Effect of monosaccharids and lectins on activity of noncyclic electron transport in chloroplasts (Hill reaction) was investigated in infected plants, with the following pathogenic systems being used: wheat and wheat x couch grass hybrids, infected with a casual organism of powdery mildew Eiysiphe graminis f. sp. tritici Marchal; wheat, infected with Fusarium sp.; necrotic and susceptible varieties of tobacco, inoculated with tobacco mosaic virus. Lectins (phytohemagglutinin, extracted from bean, and concanavalin A) and monosaccharids - ligands of exogenous and endogenous lectins (glucose, galactose, D-glucosamine, N-acetyl-D-glucosamine) - were added to the reaction mixture. In the lack of additives the activity of Hill reaction slightly increased in 1-3 days after infection and then decreased down to the control level. At the same time a transitory rise of non-cyclic electron transport in chloroplasts was observed in all the pathogenic systems with addition of N-acetyl-D-glucosamine. Addition of lectins generally did leveling these changes.