Map-based cloning of a disease resistance gene from tomato

Resistance to Pseudomonas syringae pv. tomato (Pst) in tomato is conferred by a single dominant locus, Pto (Pitblado et al., 1984 Martin et al., 1993a). The Pto locus interacts in a gene-for-gene fashion with an avirulence gene, avrPto, that has been isolated from Pst (Ronald et al., 1992). As with...

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Main Authors Martin, G.B, Brommonschenkel, S.H, Chunwongse, J, Frary, A, Ganal, M.W, Spivey, R, Wu, T, Earle, E.D, Tanksley, S.D
Format Conference Proceeding
LanguageEnglish
Published Cali (Colombia) CIAT 1994
Subjects
CARTE GENETIQUE
CLONACION
CLONAGE
LYCOPERSICON ESCULENTUM
MAPAS GENETICOS
PSEUDOMONAS SYRINGAE
RESISTANCE AUX MALADIES
RESISTENCIA A LA ENFERMEDAD
TOMATE
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Abstract Resistance to Pseudomonas syringae pv. tomato (Pst) in tomato is conferred by a single dominant locus, Pto (Pitblado et al., 1984 Martin et al., 1993a). The Pto locus interacts in a gene-for-gene fashion with an avirulence gene, avrPto, that has been isolated from Pst (Ronald et al., 1992). As with most disease resistance loci, nothing was known of the products that may be encoded at the Pto locus and this precluded standard methods of gene isolation. In order to isolate Pto, a multi-step "map-based cloning" strategy was used.
AbstractList Resistance to Pseudomonas syringae pv. tomato (Pst) in tomato is conferred by a single dominant locus, Pto (Pitblado et al., 1984 Martin et al., 1993a). The Pto locus interacts in a gene-for-gene fashion with an avirulence gene, avrPto, that has been isolated from Pst (Ronald et al., 1992). As with most disease resistance loci, nothing was known of the products that may be encoded at the Pto locus and this precluded standard methods of gene isolation. In order to isolate Pto, a multi-step "map-based cloning" strategy was used.
Author Brommonschenkel, S.H
Martin, G.B
Ganal, M.W
Tanksley, S.D
Spivey, R
Chunwongse, J
Frary, A
Wu, T
Earle, E.D
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  fullname: Earle, E.D
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  fullname: Tanksley, S.D
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Snippet Resistance to Pseudomonas syringae pv. tomato (Pst) in tomato is conferred by a single dominant locus, Pto (Pitblado et al., 1984 Martin et al., 1993a). The...
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SubjectTerms CARTE GENETIQUE
CLONACION
CLONAGE
LYCOPERSICON ESCULENTUM
MAPAS GENETICOS
PSEUDOMONAS SYRINGAE
RESISTANCE AUX MALADIES
RESISTENCIA A LA ENFERMEDAD
TOMATE
Title Map-based cloning of a disease resistance gene from tomato
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