Constructing a preliminary wheat genetic map using RGA and AFLP markers

• Published in: International journal of agriculture and biology Vol. 9; no. 6; pp. 863 - 867
• Main Authors: Fayyaz, E. (University of Tehran (Iran). Dept. of Agronomy), Shahnejat-Bushehri, A.A. (University of Tehran (Iran). Dept. of Agronomy), Tabatabaei, B.E.S. (Isfahan Univ. of Technology (Iran). Dept. of Biotechnology), Adel, J
• Format: Journal Article
• Language: English
• Published: 01.11.2007
• Subjects: DNA; ELECTROPHORESIS; GENETIC MAPS; GENETIC MARKERS; PCR; RFLP; TRITICUM AESTIVUM
• ISSN: 1560-8530

Scientists are constructing genetic linkage maps composed of DNA markers for a wide range of plant species. In this work, use of Resistance gene analogue (RGA) and Amplified fragment length polymorphism (AFLP) markers in a doubled haploid population derived from a cross between two parents named 0734 and F1054 (with com & wheat crossing method) has been investigated in order to construct a primary genetic map. In total 107 individuals were selected from a population. Mean percentage of RGA, AFLP and both of two markers polymorphism was obtained at the rate of 26.8%, 28.6% and 26.8%, respectively. Data indicated that %56.8 of markers (54 markers) could be placed in 16 linkage groups, whereas 43.2% of them (41 markers) remained un-linked.