In vitro screening for resistance to Radopholus similis Thorne (1949) in selected Philippine musa cultivars
Four Philippine musa cultivars namely 'Senorita', 'Pamoti-On', 'Matavia' and Pisang Lemak manis' were screened under in vitro conditions for resistance to Radopholus similis population from Uganda. The cultivar 'Grand Naine' was used as the susceptible ch...
Saved in:
Published in | Journal of Tropical Plant Pathology (Philippines) Vol. 40; no. 1-2 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
01.01.2004
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | Four Philippine musa cultivars namely 'Senorita', 'Pamoti-On', 'Matavia' and Pisang Lemak manis' were screened under in vitro conditions for resistance to Radopholus similis population from Uganda. The cultivar 'Grand Naine' was used as the susceptible check. Plantlets were grown in glass jars containing rooting medium for four weeks. They were inoculated with 25 manually picked gravid females of R. similis from monoxenic cultures in Alfalta callus. Ten replicates were prepared for each cultivars. They were incubated at 28 deg C with 16-hour photoperiod. After eight weeks, nematodes were extracted both from the root system and medium by maceration-sieving method. Volume of suspension was adjusted to 50 ml and the number of juveniles, males, females, separately determined based on 3 x 2 ml sub samples. Final population of R. similis was significantly lower in cultivars 'Senorita' and 'Pamoti-On' than in 'Grand Naine', while 'Matavia' and 'Pisang Lemak Manis' had nematode counts not significantly different from the susceptible check. This present in vitro screening study confirmed the resistance of the two cultivars which was previously detected under greenhouse experiments, and further established the suitability of 'Grand Naine' as a susceptible reference cultivar for Musa screening studies. |
---|---|
AbstractList | Four Philippine musa cultivars namely 'Senorita', 'Pamoti-On', 'Matavia' and Pisang Lemak manis' were screened under in vitro conditions for resistance to Radopholus similis population from Uganda. The cultivar 'Grand Naine' was used as the susceptible check. Plantlets were grown in glass jars containing rooting medium for four weeks. They were inoculated with 25 manually picked gravid females of R. similis from monoxenic cultures in Alfalta callus. Ten replicates were prepared for each cultivars. They were incubated at 28 deg C with 16-hour photoperiod. After eight weeks, nematodes were extracted both from the root system and medium by maceration-sieving method. Volume of suspension was adjusted to 50 ml and the number of juveniles, males, females, separately determined based on 3 x 2 ml sub samples. Final population of R. similis was significantly lower in cultivars 'Senorita' and 'Pamoti-On' than in 'Grand Naine', while 'Matavia' and 'Pisang Lemak Manis' had nematode counts not significantly different from the susceptible check. This present in vitro screening study confirmed the resistance of the two cultivars which was previously detected under greenhouse experiments, and further established the suitability of 'Grand Naine' as a susceptible reference cultivar for Musa screening studies. |
Author | Waele, D.E.,Philippines Univ. Los Banos, College, Laguna (Philippines). Dept. of Plant Pathology Orajay, J.I Elsen, A |
Author_xml | – sequence: 1 fullname: Orajay, J.I – sequence: 2 fullname: Elsen, A – sequence: 3 fullname: Waele, D.E.,Philippines Univ. Los Banos, College, Laguna (Philippines). Dept. of Plant Pathology |
BookMark | eNqFjj0PgjAUADtoIn78BJM36mDSIojORoObMeykwkNerC3pK_x-Gdydbrgbbi4m1lmciEgqle7kUSYzsWKmp5RZdlD7NIvE-2ZhoOAdcOURLdkXNM6DRyYO2lYIwcFD165rnekZmD5kiKFonbcIG3VKTlsgC4wGq4A13Nsx6Doa7adnDVVvAg3a81JMG20YVz8uxPp6Kc75rtGu1C9PXN7zeJyTUibHJP7nvwIARCc |
ContentType | Journal Article |
DBID | FBQ |
DatabaseName | AGRIS |
DatabaseTitleList | |
Database_xml | – sequence: 1 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
DeliveryMethod | no_fulltext_linktorsrc |
ExternalDocumentID | PH2007000484 |
GroupedDBID | ALMA_UNASSIGNED_HOLDINGS FBQ OK1 |
ID | FETCH-fao_agris_PH20070004842 |
ISSN | 0115-0804 |
IngestDate | Tue Nov 07 23:07:10 EST 2023 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1-2 |
Language | English |
LinkModel | OpenURL |
MeetingName | 35. Anniversary and Annual Scientific Meeting of the Pest Management Council of the Philippines, , Iloilo City (Philippines), 16-19 Mar 2004 |
MergedId | FETCHMERGED-fao_agris_PH20070004842 |
Notes | H10 2007000484 |
ParticipantIDs | fao_agris_PH2007000484 |
PublicationCentury | 2000 |
PublicationDate | (Jan-Dec 2004) |
PublicationDateYYYYMMDD | 2004-01-01 |
PublicationDate_xml | – month: 01 year: 2004 text: (Jan-Dec 2004) |
PublicationDecade | 2000 |
PublicationTitle | Journal of Tropical Plant Pathology (Philippines) |
PublicationYear | 2004 |
SSID | ssib007761357 |
Score | 3.1304379 |
Snippet | Four Philippine musa cultivars namely 'Senorita', 'Pamoti-On', 'Matavia' and Pisang Lemak manis' were screened under in vitro conditions for resistance to... |
SourceID | fao |
SourceType | Publisher |
SubjectTerms | EXPERIMENTACION IN VITRO EXPERIMENTATION IN VITRO FILIPINAS GROWING MEDIA http://www.fao.org/aos/agrovoc#c_16131 http://www.fao.org/aos/agrovoc#c_24799 http://www.fao.org/aos/agrovoc#c_29128 http://www.fao.org/aos/agrovoc#c_3393 http://www.fao.org/aos/agrovoc#c_5112 http://www.fao.org/aos/agrovoc#c_5731 http://www.fao.org/aos/agrovoc#c_5783 http://www.fao.org/aos/agrovoc#c_6651 http://www.fao.org/aos/agrovoc#c_8038 http://www.fao.org/aos/agrovoc#c_8157 IN VITRO EXPERIMENTATION MUSA (BANANAS) MUSA (BANANES) MUSA (BANANOS) NEMATODA OUGANDA PEST RESISTANCE PHILIPPINES RACINE RADOPHOLUS SIMILIS RAICES RESISTANCE AUX ORGANISMES NUISIBLES RESISTENCIA A LAS PLAGAS ROOTS SUBSTRAT DE CULTURE SUBSTRATOS DE CULTIVO UGANDA VARIEDADES VARIETE VARIETIES |
Title | In vitro screening for resistance to Radopholus similis Thorne (1949) in selected Philippine musa cultivars |
Volume | 40 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1NT8JAEN2AXrwYjRK_MHvwoGnaFFxaOIpCwKgxBiM3sv3CqmxJWzgYf7wz3ZaWgIl6aUhJhu2-2dnXx8wsIWe24VmWo-sqd7y6yrC2p2k7uqpbOmyHuGPVsXb4_sHoPbPbYWNYKn0Vq0tiS7M_19aV_AdVuAe4YpXsH5BdGIUb8BnwhSsgDNdfYdwXytyPw0CBtQ_vo1lSJLxBIyvEJQvM8ok7wRRi3CxSIn_if_gRZgWFklzWWqyFwoAvlCg5EQf4p9RYpkg_J7OIJyfa-nOe_u2zSmQHYTBNoMYDkGJs-v8qGzuB_dxWVFR0Q_7G5VHJ2kK1xdoXsaSuvnBXJjvfaB0MYgVjSUKJptwFkdLmaapgqoHIau_xTPDVAWgQX6exliT-LQ92Sf1gBfUjFURrDRVYLytGdNkAKvPctLxyuav2Yw9F2qSknpVJ2WxidOy2cyptmkB0ZKPY7CeAfni8SD8GO2Q7nW56JZ1gl5RcsUfe-4ImDkAXDkDBAWjuADQOaO4ANHUAKh2AniP8F9QXNAOf5vNFEXy6AH-fVLudwXVPhcGN-Bj2hlHx6eoVsiEC4R4QCjufwSxYgUarySzD5J7NbPfSqNnc8DyneUgq620c_fTFMdnKUTkhmx6sLLcKNC22TpP5_AbT7EUs |
link.rule.ids | 786 |
linkProvider | FAO Food and Agriculture Organization of the United Nations |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=In+vitro+screening+for+resistance+to+Radopholus+similis+Thorne+%281949%29+in+selected+Philippine+musa+cultivars&rft.jtitle=Journal+of+Tropical+Plant+Pathology+%28Philippines%29&rft.au=Orajay%2C+J.I&rft.au=Elsen%2C+A&rft.au=Waele%2C+D.E.%2CPhilippines+Univ.+Los+Banos%2C+College%2C+Laguna+%28Philippines%29.+Dept.+of+Plant+Pathology&rft.date=2004-01-01&rft.issn=0115-0804&rft.volume=40&rft.issue=1-2&rft.externalDocID=PH2007000484 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0115-0804&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0115-0804&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0115-0804&client=summon |