Progeny Analysis and Selection of Tomato Transformants with patⅡ Gene linked to Inherent Disease Resistance Gene

• Published in: Weon'ye gwahag gi'sulji Vol. 29; no. 4
• Main Authors: Ahn, S.Y., Yeungnam University, Gyeongsan, Republic of Korea, Kang, K.K., Hankyong National University, Ansung, Republic of Korea, Yun, H.K., Yeungnam University, Gyeongsan, Republic of Korea, Park, H.G., Dongbu Hannong Co., Ltd, Ansung, Republic of Korea
• Format: Journal Article
• Language: Korean
• Published: 01.08.2011
• Subjects: fungal inoculation; SEGREGACION; SEGREGATION; two-stepwise screening method
• ISSN: 1226-8763

This study was carried out to develop a model system using selection method for disease resistant plant breeding programs using a herbicide bialaphos-resistant patII gene as a gene-based marker. Spraying bialaphos could eliminate the susceptible plants from the segregating populations such as F₂'s and thereafter. Tomato cv. Momotaro-yoke was transformed with patII gene 60 independent transformants were acquired. Total 42 transformants were analyzed in transgene copy numbers by Southern blotting and the segregation ratios for the bialaphos resistance. Statistical analysis revealed that the transgene copy numbers and the segregation ratios were not always coincided, especially having the tendency of underestimating the real numbers of the transgenes in the multicopy lines. A two-stepwise screening method was applied to select T₁ tomato plants which linked the transgenic patII to a disease resistance gene (I2 and Ve). Based on the resistant to susceptible ratios, T-20 plant was finally selected due to the estimated linkage 12-13 cM between the patII gene to the I2 gene on chromosome 11. This newly developed system could be applied to any economical crop in breeding programs.