Isolation, Characterization, and Molecular Cloning of the cDNA Encoding a Novel Phytase from Aspergillus niger 113 and High Expression in Pichia pastoris

• Published in: Journal of biochemistry and molecular biology Vol. 37; no. 3
• Main Authors: Xiong, Ai Sheng;Yao, Quan Hong;Peng, Ri He;Li, Xian;Fan, Hui Qin(Agro-Biotechnology Research Center of Shanghai Academy of Agricultural Sciences, Shanghai, China)E-mail:yaoquanhong@yahoo.com, Guo, Mei Jin;Zhang, Si Liang(East China University of Science and Technology, Shanghai, China)
• Format: Journal Article
• Language: English
• Subjects: FERMENTACION; Fermentation; FITASA; GENE; GENES; PHYTASE; Phytase production; Pichia pastoris; PRODUCCION; PRODUCTION; Recombinant DNA; Synthetic phyI1 gene

Phytases catalyze the release of phosphate from phytic acid. Phytase-producing microorganisms were selected by culturing the soil extracts on agar plates containing phytic acid. Two hundred colonies that exhibited potential phytase activity were selected for further study. The colony showing the hig...