Isolation, Characterization, and Molecular Cloning of the cDNA Encoding a Novel Phytase from Aspergillus niger 113 and High Expression in Pichia pastoris

• Published in: Journal of biochemistry and molecular biology Vol. 37; no. 3
• Main Authors: Xiong, Ai Sheng;Yao, Quan Hong;Peng, Ri He;Li, Xian;Fan, Hui Qin(Agro-Biotechnology Research Center of Shanghai Academy of Agricultural Sciences, Shanghai, China)E-mail:yaoquanhong@yahoo.com, Guo, Mei Jin;Zhang, Si Liang(East China University of Science and Technology, Shanghai, China)
• Format: Journal Article
• Language: English
• Subjects: FERMENTACION; Fermentation; FITASA; GENE; GENES; PHYTASE; Phytase production; Pichia pastoris; PRODUCCION; PRODUCTION; Recombinant DNA; Synthetic phyI1 gene
• ISSN: 1225-8687

Phytases catalyze the release of phosphate from phytic acid. Phytase-producing microorganisms were selected by culturing the soil extracts on agar plates containing phytic acid. Two hundred colonies that exhibited potential phytase activity were selected for further study. The colony showing the highest phytase activity was identified as Aspergillus niger and designated strain 113. The phytase gene from A. niger 113 (phyI1) was isolated, cloned, and characterized. The nucleotide and deduced amino acid sequence identity between phyI1 and phyA from NRRL3135 were 90% and 98%, respectively.