Molecular Cloning and Expression Pattern Analyses of Heat Shock Protein 70 Genes from Nicotiana tabacum
We have isolated three genomic clones that code tobacco HSP70s, using a tobacco hsp70 cDNA clone as a probe. NtHSP70-1, NtHSP70-2, and NtHSP70-3 contain full open reading frames of 653, 653, and 648 amino acid residues, respectively. All share three conserved regions, namely the C-terminal substrate...
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| Published in | Journal of plant biology = Singmul Hakhoe chi Vol. 47; no. 2 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Subjects | |
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| Abstract | We have isolated three genomic clones that code tobacco HSP70s, using a tobacco hsp70 cDNA clone as a probe. NtHSP70-1, NtHSP70-2, and NtHSP70-3 contain full open reading frames of 653, 653, and 648 amino acid residues, respectively. All share three conserved regions, namely the C-terminal substrate binding domain, oligomerization domain, and N-terminal ATPase domain. In a comparison of their amino acid sequences, NtHSP70-1 and NtHSP70-2 were very similar to each other, while NtHSP70-3 showed significant differences, instead being highly homologous to the cytosolic HSP70 members of Arabidopsis thaliana and other plant species. |
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| AbstractList | We have isolated three genomic clones that code tobacco HSP70s, using a tobacco hsp70 cDNA clone as a probe. NtHSP70-1, NtHSP70-2, and NtHSP70-3 contain full open reading frames of 653, 653, and 648 amino acid residues, respectively. All share three conserved regions, namely the C-terminal substrate binding domain, oligomerization domain, and N-terminal ATPase domain. In a comparison of their amino acid sequences, NtHSP70-1 and NtHSP70-2 were very similar to each other, while NtHSP70-3 showed significant differences, instead being highly homologous to the cytosolic HSP70 members of Arabidopsis thaliana and other plant species. |
| Author | Cho, E.K.;Hong, C.B.(Seoul National University, Seoul, Republic of Korea)E-mail:hcb@snu.ac.kr |
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| Snippet | We have isolated three genomic clones that code tobacco HSP70s, using a tobacco hsp70 cDNA clone as a probe. NtHSP70-1, NtHSP70-2, and NtHSP70-3 contain full... |
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| SubjectTerms | abiotic stress ESTRES expression pattern genomic clones heat shock protein 70 HORMONAS HORMONE hormone response HORMONES Nicotiana tabacum STRESS |
| Title | Molecular Cloning and Expression Pattern Analyses of Heat Shock Protein 70 Genes from Nicotiana tabacum |
| Volume | 47 |
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