Studies on the Mobilization of a Maize Transposable Family, Ac/Ds, in Pepper Using In Vivo Transient Assay System

• Published in: Journal of plant biology = Singmul Hakhoe chi Vol. 47; no. 1
• Main Author: Kim, C.M.;Je, B.I.;Koo, J.C.;Piao, Hai Long;Park, S.J.;Jeon, J.M.;Kim, M.K.;Park, S.H.;Park, J.Y.;Lee, E.J.;Chung, W.S.;Lee, K.H.;Kang, K.Y.;Lee, S.H.;Han, C.D.(Gyeongsang National University, Jinju, Republic of Korea)E-mail:cdhan@nongae.gsnu.ac.kr
• Format: Journal Article
• Language: English
• Subjects: Ac/Ds; pepper; PIMIENTA; POIVRE; transient assay
• ISSN: 1226-9239

A maize transposable family, Ac/Ds, has been successfully utilized as a mutagenizing agent not only in monocot but also in dicot. In order to develop insertional mutagenesis system in pepper, the mobility of Ac/Ds has been examined. In this study, the excision of the elements was monitored via transient assay system with protoplasts. Two different systems were developed and comparedone- and two-elements systems. In a one-element system, Ac alone was introduced into cells. As a two-element system, Ac and Ds were cloned into a single vector and were expressed in protoplasts.