In vitro glutathione production using mixed cells in an aerated slurry bioreactor

• Published in: Korean Journal of Biotechnology and Bioengineering Vol. 14; no. 4
• Main Authors: Koh, S.Y, Koo, Y.M. (Inha University, Inchon (Korea Republic). Department of Biological Engineering)
• Format: Journal Article
• Language: Korean
• Published: 01.08.1999
• Subjects: BIOLOGICAL PRODUCTION; ESTIERCOL FLUIDO; GLULATHIONE; LISIER; PRODUCCION BIOLOGICA; PRODUCTION BIOLOGIQUE; SLURRY; STATER CULTURES; SURFACE ACTIVE AGENTS; SURFACTANT; SURFACTANTES
• ISSN: 1225-7117

Glutathione production was carried out using mixed cells of E. coli TG1/pDG7 alpha and bakers yeast in an Aerated Slurry Bioreactor. Glutathione-producing enzymes were stable for 34 hours, yielding 4.6 mM glutathione in suspension reaction. Glutathione production with high density mixed cells was studied as a function of flow rate in an Aerated Slurry Bioreactor. Glutathione production concentration was higner than that in suspension rection for 32 hours at the substrate feeding rate of 5.2 mL/hr with cell recycle in continuous Aerated Slurry Bioreactor. It was for 42 hours at 2.6 mL/hr and 22 hours at 5.2 mL/hr without cell recycle. Glutathone productivity was 25.7 mg/g wet cell.hr at the substrate feeding rate of 10.4 mL/hr with cell recycle, but 5.28 mg/g wet cell.hr at 5.2 mL/hr and 1.65 mg/g wet cell.hr at 2.6 mL/hr without cell recycle. Effective production time increased from 25 to 45 hours, by using a surfactant, tween 80. As a purfing gas, nitrogen was tested instead of air to avoid a possible oxidizing effect on glutathione-producing enzymes, resulting in the increases of effective production time to 40 hours.