Saving resources: Avian influenza surveillance using pooled swab samples and reduced reaction volumes in real-time RT-PCR

• Published in: Journal of virological methods Vol. 186; no. 1-2
• Main Authors: Fereidouni S.R, Harder T.C, Gaidet N, Ziller M, Hoffmann B, Hammoumi S, Globig A, Starick E
• Format: Journal Article
• Language: English
• Published: 2012
• Subjects: ALLEMAGNE; ANIMAL SAUVAGE; COUT; ECHANTILLONNAGE; EXCRETION; FRANCE; INFLUENZAVIRUS AVIAIRE; OISEAU; SURVEILLANCE EPIDEMIOLOGIQUE; TECHNIQUE ANALYTIQUE

The occurrence of highly pathogenic (HP) avian influenza (AI) H5N1 in Asia and its spread to Africa and Europe prompted costly monitoring programs of wild birds and domestic poultry. AI virus excretion is tested by examining avian swab samples by real-time reverse transcription PCR (RT-qPCR). In thi...