The Pichia pastoris HIS4 gene: nucleotide sequence, creation of a non-reverting his4 deletion mutant, and development of HIS4-based replicating and integrating plasmids

• Published in: Current genetics Vol. 265
• Main Authors: Crane, D.I, Gould, S.J. (Kennedy Krieger Research Inst., Baltimore (USA))
• Format: Journal Article
• Language: English
• Published: 1995
• Subjects: GENE; GENES; HIDROLASAS; HYDROLASE; MARCADORES GENETICOS; MARQUEUR GENETIQUE; MUTACION INDUCIDA; MUTATION PROVOQUEE; OXIDORREDUCTASAS; OXYDOREDUCTASE; PICHIA; PLASMIDE; PLASMIDIOS; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE

A clone of the Pichia pastoris HIS4 gene was obtained and its nucleotide sequence was determined. Based upon its deduced amino-acid sequence, the product of the P. pastoris HIS4 gene has the same structural organization as the Saccharomyces cerevisiae His4 protein and appears to encode a trifunction...