Agrobacterium tumefaciens mediated genetic transformation of Momordica charantia L

• Published in: Journal of Fujian Agriculture and Forestry University Vol. 36; no. 2
• Main Author: Lin Yizhang,Luo Yanhua
• Format: Journal Article
• Language: Chinese
• Published: 01.03.2007
• Subjects: AGROBACTERIUM; GENETIC TRANSFORMATION; http://www.fao.org/aos/agrovoc#c_206; http://www.fao.org/aos/agrovoc#c_3220; http://www.fao.org/aos/agrovoc#c_4903; MOMORDICA CHARANTIA; TRANSFORMACION GENETICA; TRANSFORMATION GENETIQUE

用含质粒载体pB I121的根癌农杆菌转化“南屿”苦瓜子叶节,通过对gus基因瞬时表达情况进行观测,对根癌农杆菌介导的苦瓜遗传转化体系相关因素进行研究.结果表明,苦瓜子叶节必须预培养3 d左右,用D600nm值为0.3-0.5之间的菌液浸泡,侵染时间为10 min,然后共培养3-4 d.再生植株经PCR检测初步证明gus基因已成功整合到苦瓜基因组中....