Determination of dimethyl hydantion in compound bromo-chloro-dimethyl hydantion disinfectant by high performance liquid chromatography

• Published in: Journal of Fujian Agriculture and Forestry University Vol. 34; no. 3
• Main Author: Chen Qiang, Li Qinglu, Li Jiansheng
• Format: Journal Article
• Language: Chinese
• Published: 01.09.2005
• Subjects: ANALISIS CUANTITATIVO; ANALYSE QUANTITATIVE; DESINFECTANT; DESINFECTANTES; DISINFECTANTS; HPLC; http://www.fao.org/aos/agrovoc#c_2331; http://www.fao.org/aos/agrovoc#c_24469; http://www.fao.org/aos/agrovoc#c_32660; QUANTITATIVE ANALYSIS

采用高效液相色谱法测定二甲基海因含量，色谱条件：Symmetry十八烷基键合硅胶柱（4．6mm×75mm,HPLC column）；流动相：甲醇＋0.02mol・L. 1KH2PO4（15：85）；检测波长206nm，流速1mL・min. 1,二甲基海因的质量浓度为8-50μ・mL. 1时二甲基海因含量与色谱峰面积呈良好的线性关系（线性方程：Y=1．07×10. X＋1．81×10. ，回归系数，r=0.9999）,检测限为3μg・mL. 1，方法回收率达97．74％-99．89％，说明这种方法可用于复方溴氯海因消毒剂和相关产品中二甲基海因含量的定量测定．...