Immunohistochemical distribution of prolactin containing cells in the pituitary of the chickens

• Published in: Veterinární medicína Vol. 50; no. 5
• Main Author: Erdost, H.(Uludag Univ., Bursa (Turkey). Dept. of Histology - Embryology)E-mail:edost@uludag.edu.tr
• Format: Journal Article
• Language: English
• Subjects: ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; CELLS; CELLULE; CELULAS; CHICKS; COCKERELS; COQUELET; FEMALES; FEMELLE; GALLITO; GLANDULA PITUITARIA; HEMBRA; HYPOPHYSE; IMMUNOLOGICAL TECHNIQUES; MACHO; MALE; MALES; MONOCLONAL ANTIBODIES; PITUITARY GLAND; POLLITO; POUSSIN; PROLACTIN; PROLACTINA; PROLACTINE; TECHNIQUE IMMUNOLOGIQUE; TECNICAS INMUNOLOGICAS; ULTRAESTRUCTURA; ULTRASTRUCTURE
• ISSN: 0375-8427

The objective of this study was to determine the immunohistochemical distribution and density of prolactin containing cells in the pituitary gland of female and male chickens after hatching during five months. The modified labelled avidin-biotin method with monoclonal mouse antihuman prolactin as a primary antibody was used to detect prolactin positive cells. At the end of each month, prolactin containing cells were identified in the pars distalis of the pituitary glands. Males had less prolactin positive cells than females. The number of these cells increased with increasing age in both males and females, whereas the reaction density decreased slightly.