Micropropagation of an endangered orchid Anoectochilus formosanus

• Published in: Biologia plantarum Vol. 48; no. 3
• Main Authors: Ket, N.V.(Chungbuk National Univ., Cheong-ju (Korea). Research Center for the Development of Advanced Horticultural Technology), Hahn, E.J.(Chungbuk National Univ., Cheong-ju (Korea). Research Center for the Development of Advanced Horticultural Technology), Park, S.Y.(Chungbuk National Univ., Cheong-ju (Korea). Research Center for the Development of Advanced Horticultural Technology), Chakrabarty, D.(Chungbuk National Univ., Cheong-ju (Korea). Research Center for the Development of Advanced Horticultural Technology), Paek, K.Y.(Chungbuk National Univ., Cheong-ju (Korea). Research Center for the Development of Advanced Horticultural Technology)E-mail:paekky@chungbuk.ac.kr
• Format: Journal Article
• Language: English
• Subjects: 6-BENZILADENINA; 6-BENZYLADENINE; ACTIVATED CARBON; ADAPTACION; ADAPTATION; ANOECTOCHILUS FORMOSANUS; AZUCAR; BROTES; CARBON ACTIVADO; CHARBON ACTIF; CRECIMIENTO; CROISSANCE; CULTIVO DE TEJIDOS; CULTURE DE TISSU; CULTURE MEDIA; ENDANGERED SPECIES; ESPECE EN DANGER; ESPECIES EN PELIGRO DE EXTINCION; EXPLANT; EXPLANTES; EXPLANTS; FEUILLE; FLORACION; FLORAISON; FLOWERING; GROWTH; HOJAS; KINETIN; KINETINE; LARGURA; LEAVES; LENGTH; LONGUEUR; MEDIO DE CULTIVO; MICROPROPAGACION; MICROPROPAGATION; MILIEU DE CULTURE; ORCHIDACEAE; PESO; POIDS; POUSSE; QUINETINA; SACCHAROSE; SHOOTS; SUCRE; SUCROSA; SUCROSE; SUGAR; THIDIAZURON; TIDIAZURON; TISSUE CULTURE; WEIGHT
• ISSN: 0006-3134; 1573-8264

A rapid and efficient procedure is outlined for in vitro clonal propagation of an elite cultivar of jewel orchid. Multiple shoot proliferation was induced in shoot tip explants on Hyponex media supplemented with 1 mg/cubic dm benzyladenine or 1-2 mg/cubic dm thidiazuron (TDZ). Addition of activated charcoal (1 g/cubic dm) to the TDZ containing medium promoted multiple shoot formation (11.1 shoots per explant). However, the regenerated shoots had slow growth rate and failed to elongate. This problem was overcome by transferring the shoot clumps to a hormone free Hyponex medium supplemented with 2% sucrose and 0.5 g/cubic dm activated charcoal. Rooting was induced in 100% of the regenerated shoots in the same media. The plantlets were acclimatized and established in greenhouse.