Genome induced mutation in Callistephus chinensis, 2: Results of colchicine application - efficiency of polyploidisation

• Published in: Horticultural science (Praha) Vol. 28; no. 1
• Main Authors: Hanzelka, P, Kobza, F. (Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Zahradnicka Fakulta)
• Format: Journal Article
• Language: English
• Published: 01.03.2001
• Subjects: ANALISIS HISTOCITOLOGICO; ANALYSE HISTOCYTOLOGIQUE; CALLISTEPHUS CHINENSIS; COLCHICINA; COLCHICINE; FEUILLE; HISTOCYTOLOGICAL ANALYSIS; HOJAS; INDICE DE SUPERFICIE FOLIAR; INDICE DE SURFACE FOLIAIRE; INDUCED POLYPLOIDY; LEAF AREA INDEX; LEAVES; POLIPLOIDIA INDUCIDA; POLYPLOIDIE PROVOQUEE; VARIEDADES; VARIETE; VARIETIES
• ISSN: 0862-867X

Considerable changes morphology of leaf blade can be considered as an additional mark of polyploid plants. It manifested itself by a changed value of leaf index. Leaf index (length and width leaf blade ratio) of diploid plants was closely around 1.5, in the case of polyploid plants it was around 1.05-1.1. Leaves were more rounded and thicker with more entire leaf blade. The primary selection of probably polyploid plants was performed on the base of these morphological changes. Plants chosen in the primary selection were analysed by flow-cytometry. From obtained results, the number of polyploidy (tetraploidy and mixoploidy) plants was determined in population C0 of Callistephus chinensis. The efficiency of polyploidisation is based on the number and on the survival rate of polyploid plants.