Preparation of monoclonal antibodies against avian influenza virus and analysis of anti-polypeptides antigens

以纯化的H9N2亚型禽流感病毒为抗原,免疫BALB/c小鼠,细胞融合后,经间接ELISA和血凝抑制试验(HI)筛选,获得了8株能稳定分泌抗禽流感病毒单克隆抗体的杂交瘤细胞株.特异性试验证明,8株杂交瘤细胞株诱生小鼠腹水的特异ELISA抗体效价可达1∶3.2×103～1∶5.1×106,其中2株HI效价达212.8株单抗与H5亚型血凝素分型抗原不发生血凝,与减蛋综合征(EDS-76)病毒、传染性支气管炎病毒(IBV)、新城疫病毒(NDV)均不反应.亚类鉴定证实,除1C7单抗为IgG2b外,其他7株均为IgG1亚类.Western blotting试验分析初步表明,8株单抗至少针对纯化病毒粒子3种...

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Published inZhongguo shouyi xuebao Vol. 27; no. 1
Main Authors Wu Renwei, Xiao Yuncai, Hu Sishun
Format Journal Article
LanguageChinese
Published 01.06.2007
Subjects
ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
ANTIGENE
ANTIGENOS
ANTIGENS
AVIAN INFLUENZAVIRUS
http://www.fao.org/aos/agrovoc#c_24051
http://www.fao.org/aos/agrovoc#c_504
http://www.fao.org/aos/agrovoc#c_5691
http://www.fao.org/aos/agrovoc#c_9017
INFLUENZAVIRUS AVIAIRE
MONOCLONAL ANTIBODIES
PEPTIDE
PEPTIDES
PEPTIDOS
VIRUS DE LA INFLUENZA AVIAR
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Abstract 以纯化的H9N2亚型禽流感病毒为抗原,免疫BALB/c小鼠,细胞融合后,经间接ELISA和血凝抑制试验(HI)筛选,获得了8株能稳定分泌抗禽流感病毒单克隆抗体的杂交瘤细胞株.特异性试验证明,8株杂交瘤细胞株诱生小鼠腹水的特异ELISA抗体效价可达1∶3.2×103～1∶5.1×106,其中2株HI效价达212.8株单抗与H5亚型血凝素分型抗原不发生血凝,与减蛋综合征(EDS-76)病毒、传染性支气管炎病毒(IBV)、新城疫病毒(NDV)均不反应.亚类鉴定证实,除1C7单抗为IgG2b外,其他7株均为IgG1亚类.Western blotting试验分析初步表明,8株单抗至少针对纯化病毒粒子3种不同的蛋白抗原,其中3株针对核蛋白(NP),2株针对基质蛋白M1,2株针对血凝素HA/HA1.对感染细胞的Western blotting分析结果与纯化病毒结果基本一致,其中1株未明显沉淀纯化病毒粒子蛋白的单抗可以与感染细胞的M2蛋白多肽反应。
AbstractList 以纯化的H9N2亚型禽流感病毒为抗原,免疫BALB/c小鼠,细胞融合后,经间接ELISA和血凝抑制试验(HI)筛选,获得了8株能稳定分泌抗禽流感病毒单克隆抗体的杂交瘤细胞株.特异性试验证明,8株杂交瘤细胞株诱生小鼠腹水的特异ELISA抗体效价可达1∶3.2×103～1∶5.1×106,其中2株HI效价达212.8株单抗与H5亚型血凝素分型抗原不发生血凝,与减蛋综合征(EDS-76)病毒、传染性支气管炎病毒(IBV)、新城疫病毒(NDV)均不反应.亚类鉴定证实,除1C7单抗为IgG2b外,其他7株均为IgG1亚类.Western blotting试验分析初步表明,8株单抗至少针对纯化病毒粒子3种不同的蛋白抗原,其中3株针对核蛋白(NP),2株针对基质蛋白M1,2株针对血凝素HA/HA1.对感染细胞的Western blotting分析结果与纯化病毒结果基本一致,其中1株未明显沉淀纯化病毒粒子蛋白的单抗可以与感染细胞的M2蛋白多肽反应。
Author Wu Renwei
Xiao Yuncai
Hu Sishun
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SubjectTerms ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
ANTIGENE
ANTIGENOS
ANTIGENS
AVIAN INFLUENZAVIRUS
http://www.fao.org/aos/agrovoc#c_24051
http://www.fao.org/aos/agrovoc#c_504
http://www.fao.org/aos/agrovoc#c_5691
http://www.fao.org/aos/agrovoc#c_9017
INFLUENZAVIRUS AVIAIRE
MONOCLONAL ANTIBODIES
PEPTIDE
PEPTIDES
PEPTIDOS
VIRUS DE LA INFLUENZA AVIAR
Title Preparation of monoclonal antibodies against avian influenza virus and analysis of anti-polypeptides antigens
Volume 27
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