Studies of transforming 2 RAPD markers based on F2 population from B.oleracea var.capitata×B.oleracea var.alboglabra into ERPAD markers

• Published in: Xi bei nong lin ke ji da xue xue bao.Zi ran ke xue ban Vol. 33; no. 4
• Main Author: Chen Shuxia, Wang Xiaowu, Cheng Zhihui
• Format: Journal Article
• Language: Chinese
• Published: 01.04.2005
• Subjects: BRASSICA OLERACEA CAPITATA; GENETIC MARKERS; GENETIC TRANSFORMATION; HIBRIDOS; http://www.fao.org/aos/agrovoc#c_24030; http://www.fao.org/aos/agrovoc#c_3220; http://www.fao.org/aos/agrovoc#c_36341; http://www.fao.org/aos/agrovoc#c_3707; http://www.fao.org/aos/agrovoc#c_5975; http://www.fao.org/aos/agrovoc#c_9404; HYBRIDE; HYBRIDS; MARCADORES GENETICOS; MARQUEUR GENETIQUE; PLANT POPULATION; POBLACION VEGETAL; POPULATION VEGETALE; RAPD; TRANSFORMACION GENETICA; TRANSFORMATION GENETIQUE
• ISSN: 1671-9387

在对芥蓝C100-12×甘蓝秋50-Y7杂交组合构建F2作图群体进行RAPD检测的基础上，分别对4个O12,R13,Q14,P17进行了延长1个碱基的研究，其中O12和P17能将部分特异RAPD标记转化为稳定性更强的ERPAD标记，但对R13和Q14随机引物未找到相应的ERPAD标记。 Four random primers O12,R13,Q14,P17 were researched by screening 3′-end-extended random primers,in order to enhance the stability and specificity of RAPD markers of F2 population from B.oleracea var. capitata×B.oleracea var. alboglabra.The stability of ERPAD marker was higher than that of the original RAPD markers of O12 and P17,However,such ERPAD marker was not derived from R13 and Q14.