Identification a Novel Raw-Starch-Degrading-α-Amylase from a Tropical Marine Bacterium
Problem statement: Bacteria from the surface of the tropical marine hard coral Acropora sp. were screened for producing raw-starch-degrading-᭡mylase. Approach: Based on its 16s rDNA sequence, a bacterium that produced the highest amylolitic activity was identified as Bacillus amyloliquifac...
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Main Authors | , , , |
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Format | Publication |
Language | English |
Published |
Science Publications
2010
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Summary: | Problem statement: Bacteria from the surface of the tropical marine hard coral Acropora sp. were screened for producing raw-starch-degrading-᭡mylase. Approach: Based on its 16s rDNA sequence, a bacterium that produced the highest amylolitic activity was identified as Bacillus amyloliquifaciens ABBD. The bacterial isolate secreted a ᭡mylase extracellularly and then the enzyme was partially purified by ammonium sulfate precipitation followed by anion exchange chromatography. Results: Electrophoresis results both SDS-PAGE and native PAGE suggested that the enzyme was a heterodimeric protein (97 kDa) consisting of 45 and 55 kDa subunits. The ᭡mylase had an optimum pH of 7.0 and temperature of 60C. More than 80% activity of the enzyme was retained under high salt conditions (up to 20% NaCl). The enzyme remained stable at 50C for 1 h. Starch hydrolysis by the enzyme at 70C yielded oligosaccharides (G2-G4) and at room temperature yielded glucose/maltose (G1 and G2). Conclusion: The B. amyloliquifaciens ABBD ᭡mylase was capable of degrading various raw starch granules from corn, rice, cassava and sago at room temperature. |
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Bibliography: | http://www.thescipub.com/pdf/10.3844/ajbbsp.2010.300.306 http://www.doaj.org/doaj?func=openurl&genre=article&issn=15533468&date=2010&volume=6&issue=4&spage=300 |