METHOD FOR SIMULTANEOUS DETECTION OF MULTIPLE FLUOROPHORES FOR IN SITU HYBRIDIZATION AND CHROMOSOME PAINTING

• Main Authors: CABIB, DARIO, BUCKWALD, ROBERT, A, GARINI, YUVAL, KATZIR, NIR, LAVI, MOSHE, WINE, DAVID, SOENKSEN, DIRK, G
• Format: Patent
• Language: English; French
• Published: 26.06.1997
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CALCULATING; CHEMISTRY; COLORIMETRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPUTING; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; COUNTING; DIAGNOSIS; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; IDENTIFICATION; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT,POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED,VISIBLE OR ULTRA-VIOLET LIGHT; MEASURING; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS; MEASURING LENGTH, THICKNESS OR SIMILAR LINEARDIMENSIONS; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; RADIATION PYROMETRY; SPIRITS; SURGERY; TESTING; VINEGAR; WINE

A spectral imaging method for detecting and analyzing fluorescent in situ hybridizations (fig. 5) employing numerous chromosome paints (fig. 9) and/or loci specific probes each labeled with a different fluorophore or a combination of fluorophores, the method is highly sensitive both in spatial and spectral resolutions (fig. 6) and is capable of simultaneous detection of dozens of fluorophores or combinations of fluorophores (fig. 7). The method of the present invention can be used for the detection of fluorescently painted complete sets of chromosomes and/or multiple loci from a species such as human (fig. 10). Cette invention concerne un procédé d'imagerie spectrale permettant de détecter et d'analyser des hybridations fluorescentes in situ (fig.5) faisant usage de nombreux colorants (fig. 9) de chromosomes et/ou de sondes spécifiques de certains loci, marquées chacune par un fluorophore différent ou une combinaison de fluorophores. Ledit procédé est très sensible à la fois au niveau des répartitions spatiale et spectrale (fig. 6) et il permet la détection simultanée de douzaines de fluorophores ou de combinaisons de fluorophores (fig. 7). Le procédé de la présente invention peut servir à la détection d'ensembles complets de chromosomes colorés de manière fluorescente et/ou de multiples loci issus d'une espèce telle que l'espèce humaine (fig. 10).