GENE FUSION DETECTION METHOD AND APPARATUS

• Main Authors: YANG, Zhi, CHEN, Yanmei, SUN, Jiguo, ZHAO, Duojun
• Format: Patent
• Language: Chinese; English; French
• Published: 16.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Provided are a gene fusion detection method and apparatus. A gene fusion detection method, the method comprising: (1) taking a genomic DNA fragment of a sample to be detected, and adding a linker to the end of the DNA fragment; (2) designing a PCR primer according to a linker sequence and a sequence located upstream or downstream of a target region, and performing PCR amplification by using the DNA fragment obtained in step (1) as a template so as to obtain a PCR product; (3) sequencing the PCR product obtained in step (2) by using a nanopore sequencing method; (4) comparing the sequencing result obtained in step (3) to a reference genome sequence so as to determine whether gene fusion occurs in the target region of the sample. Also provided is a gene fusion detection apparatus. The provided method can accurately reflect information of a long read length sequence, and is advantageous in detecting fused genes at a fusion breakpoint located in a repeated sequence region. La présente invention concerne un procéd