AMPLIFIABLE METABOLIC LABELING FOR DETECTION OF BACTERIAL VIABILITY, GROWTH AND ANTIBIOTIC SUSCEPTIBILITY

• Main Authors: SIEGRIST, M. Sloan, MELZER, Emily S, SANTAMARIA, Carolina, KOYASSERIL-YEHIYA, Thameez M, ROBERTS, Eugenia
• Format: Patent
• Language: English; French
• Published: 09.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

Provided herein is a rapid amplifiable readout of bacterial growth, such as an ELISA paired with metabolic labeling. Methods comprise a) contacting a sample from a subject suffering from a bacterial infection with an antibiotic; and b) determining the effect, if any, of the antibiotic on incorporation of a single- or di-D-amino acid into peptidoglycan of the bacterial cell or incorporation of trehalose into the bacterial cell envelope, wherein an antibiotic that reduces, as compared to control without the antibiotic being present, incorporation of the D-amino acid into peptidoglycan of the bacterial cell or inhibited incorporation of trehalose into the bacterial cell envelope is an antibiotic that is effective. La présente invention concerne une lecture rapide amplifiable d'une croissance bactérienne, telle qu'un ELISA apparié à un marquage métabolique. Des procédés comprennent a) la mise en contact avec un antibiotique d'un échantillon provenant d'un sujet souffrant d'une infection bactérienne ; et b) la détermination de l'effet, s'il en est, de l'antibiotique sur l'incorporation d'un acide aminé unique ou di-D dans un peptidoglycane de la cellule bactérienne ou l'incorporation de tréhalose dans l'enveloppe de la cellule bactérienne. Un antibiotique qui réduit, tel que comparé à un témoin sans antibiotique, l'incorporation de l'acide D-aminé dans un peptidoglycane de la cellule bactérienne ou l'incorporation inhibée de tréhalose dans l'enveloppe de la cellule bactérienne, est un antibiotique efficace.