METHOD FOR MANUFACTURING STEM CELL SHEET

• Main Authors: KIM, Dong-Sung, PARK, Hun-Jun, LEE, Seong-Jin, CHOI, Andrew
• Format: Patent
• Language: English; French; Korean
• Published: 27.01.2022
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a method capable of manufacturing a stem cell sheet and, specifically, provides a method for manufacturing a stem cell sheet, the method comprising the steps of: seeding stem cells on one surface of a cell culture membrane, which includes a porous membrane and a cell culture layer on the surface of the porous membrane; and seeding co-culture cells on the opposite surface of the stem cell-seeded surface. La présente invention concerne un procédé capable de produire un feuillet de cellules souches et, plus particulièrement, fournit un procédé de fabrication d'un feuillet de cellules souches, le procédé comprenant les étapes suivantes : ensemencement des cellules souches sur une surface d'une membrane de culture cellulaire, qui comprend une membrane poreuse et une couche de culture cellulaire sur la surface de la membrane poreuse ; et ensemencement des cellules de co-culture sur la surface opposée de la surface ensemencée en cellules souches. 본 발명은 줄기세포 시트를 제조할 수 있는 방법에 관한 것으로, 상세하게 본 발명은 다공성 멤브레인 및 상기 다공성 멤브레인 표면의 세포 배양층을 포함하는 세포 배양용 멤브레인의 일면에 줄기세포를 접종하는 단계; 및 상기 줄기세포가 접종된 면의 반대면에 공배양 세포를 접종하는 단계를 포함하는, 줄기세포 시트를 제조하는 방법을 제공한다.