DUAL GUIDE RNA FOR CRISPR/CAS GENOME EDITING IN PLANTS CELLS

• Main Author: LHUISSIER, Franck Georges Paul
• Format: Patent
• Language: English; French
• Published: 07.05.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention pertains to a method for targeted modification of DNA in a plant cell, comprising a step of contacting the DNA with an RNA-guided CRISPR-system nuclease complex, wherein the complex comprises a crRNA anda tracrRNA as separate molecules. The invention further pertains to said RNA-guided...