DERMATOPHAGOIDES PTERONYSSINUS ALLERGEN DER P 29 GENE RECOMBINANT PROTEIN AND USE THEREOF

• Main Authors: ZHOU, Ying, WU, Guorong, QIAN, Jun, YU, Zhiwei, CHEN, Yuexin, CUI, Yubao
• Format: Patent
• Language: Chinese; English; French
• Published: 16.04.2020
• Subjects: CHEMISTRY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; TESTING

Disclosed are a Dermatophagoides pteronyssinus allergen Der p 29 gene recombinant protein and the use thereof. In the present invention, a sequence alignment is performed after transcriptome sequencing to obtain a sequence in which a fragment is highly homologous to Dermatophagoides farinae Der f 29; the fragment is amplified, and then subjected to gene cloning, expression and purification; the purified protein and the serum of a patient allergic to Dermatophagoides pteronyssinus are subjected to Western blotting; and the protein coded by the fragment is found to be highly active, wherein the sequence of the recombinant protein is shown as SEQ ID NO: 1. The sequence of the Dermatophagoides pteronyssinus Der p 29 gene is subjected to gene cloning, expression and purification to obtain the recombinant protein thereof. The recombinant protein was used for precisely detecting 16 cases of child patients with allergic asthma caused by Dermatophagoides pteronyssinus by means of Western blotting experiments, wherein