VIRUS-BASED REPLICON FOR EDITING GENOME WITHOUT INSERTING REPLICON INTO PLANT GENOME, AND USE THEREOF

• Main Authors: KIM, Jae Yean, JEONG, Se Jeong, SIVANKALYANI, Velu, KIM, Jihae, PRAMANIK, Dibyajyoti, DOAN, Thi Hai Duong, VU, Tien Van, PARK, Seo-Jin, SUNG, Yeon Woo, SON, Geon Hui, KIM, Hyun Jeong, TRAN, Mil Thi, SHELAKE, Mahadev Rahul
• Format: Patent
• Language: English; French; Korean
• Published: 19.03.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to: a recombinant vector for editing a genome without inserting a replicon into the genome in a T0 generation plant, comprising a geminivirus-based replicon between left border (LB) and right border (RB) sequences of a Ti plasmid; and a method for editing a genome without inserting a replicon into the genome in a T0 generation plant, comprising a step of transforming plant cells by inserting a foreign gene into the recombinant vector. La présente invention concerne : un vecteur recombinant permettant d'éditer un génome sans insérer de réplicon dans le génome d'une plante de génération T0, comprenant un réplicon à base de géminivirus entre les séquences de bordure gauche (LB) et de bordure droite (RB) d'un plasmide Ti ; et un procédé d'édition de génome sans insertion de réplicon dans le génome d'une plante de génération T0, comprenant une étape de transformation de cellules végétales par insertion d'un gène étranger dans le vecteur recombinant. 본 발명은 Ti 플라스미드의 LB(left border) 및 RB(right border) 서열 사이에 게미니바이러스(geminivirus) 기반 레플리콘(replicon)을 포함하는 T 0 세대 식물체에서 게놈 내 레플리콘의 삽입 없이 유전체를 교정하기 위한 재조합 벡터, 상기 재조합 벡터에 외래 유전자를 삽입하여 식물세포를 형질전환하는 단계를 포함하는 T 0 세대 식물체에서 게놈 내 레플리콘의 삽입 없이 유전체를 교정하는 방법에 관한 것이다.