METHOD FOR PREPARING DENDRITIC CELL POPULATION
The problem is to provide a method for obtaining a large amount of dendritic cells from blood cells collected from a subject. Provided is a method for preparing a dendritic cell population wherein the preparation method includes (a): a step for isolating a mononuclear cell population from a collecte...
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Format | Patent |
Language | English French Japanese |
Published |
18.01.2018
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Abstract | The problem is to provide a method for obtaining a large amount of dendritic cells from blood cells collected from a subject. Provided is a method for preparing a dendritic cell population wherein the preparation method includes (a): a step for isolating a mononuclear cell population from a collected blood cell population, (b): a step for removing a cell population that expresses CD3 on the cell surface from the mononuclear cell population isolated in step (a), and (c): a step for culturing the mononuclear cell population obtained in step (b) in a medium containing GM-CSF and SCF using a culture vessel coated by a novel coating agent.
L'invention a pour objet de fournir un procédé destiné à obtenir une grande quantité de cellules arborescentes à partir de cellules sanguines prélevées chez un sujet. Plus précisément, l'invention fournit un procédé de préparation de population de cellules arborescentes qui inclut : (a) une étape au cours de laquelle une population de mononucléaires provenant d'une population de cellules sanguines prélevées, est séparée ; (b) une étape au cours de laquelle une population de cellules exprimant un CD3 à la surface des cellules, est retirée de la population de mononucléaires séparée à ladite étape (a) ; et (c) une étape au cours de laquelle la population de mononucléaires obtenue à ladite étape (b), est cultivée dans un milieu contenant un GM-CSF et un SCF à l'aide d'un réceptacle de culture revêtu d'un nouvel agent de revêtement.
被験者から採取された血球細胞から大量の樹状細胞を得る方法を提供することを課題とする。樹状細胞集団の調製方法であって、(a):採取された血球細胞集団から単核球集団を分離するステップと、(b):前記ステップ(a)で分離された単核球集団から、CD3を細胞表面に発現する細胞集団を除去するステップと、(c):前記ステップ(b)で得られた単核球集団を、新規なコーティング剤によってコーティングされた培養容器を用い、GM-CSF及びSCFを含む培地で培養するステップ、を含む、調製方法を提供する。 |
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AbstractList | The problem is to provide a method for obtaining a large amount of dendritic cells from blood cells collected from a subject. Provided is a method for preparing a dendritic cell population wherein the preparation method includes (a): a step for isolating a mononuclear cell population from a collected blood cell population, (b): a step for removing a cell population that expresses CD3 on the cell surface from the mononuclear cell population isolated in step (a), and (c): a step for culturing the mononuclear cell population obtained in step (b) in a medium containing GM-CSF and SCF using a culture vessel coated by a novel coating agent.
L'invention a pour objet de fournir un procédé destiné à obtenir une grande quantité de cellules arborescentes à partir de cellules sanguines prélevées chez un sujet. Plus précisément, l'invention fournit un procédé de préparation de population de cellules arborescentes qui inclut : (a) une étape au cours de laquelle une population de mononucléaires provenant d'une population de cellules sanguines prélevées, est séparée ; (b) une étape au cours de laquelle une population de cellules exprimant un CD3 à la surface des cellules, est retirée de la population de mononucléaires séparée à ladite étape (a) ; et (c) une étape au cours de laquelle la population de mononucléaires obtenue à ladite étape (b), est cultivée dans un milieu contenant un GM-CSF et un SCF à l'aide d'un réceptacle de culture revêtu d'un nouvel agent de revêtement.
被験者から採取された血球細胞から大量の樹状細胞を得る方法を提供することを課題とする。樹状細胞集団の調製方法であって、(a):採取された血球細胞集団から単核球集団を分離するステップと、(b):前記ステップ(a)で分離された単核球集団から、CD3を細胞表面に発現する細胞集団を除去するステップと、(c):前記ステップ(b)で得られた単核球集団を、新規なコーティング剤によってコーティングされた培養容器を用い、GM-CSF及びSCFを含む培地で培養するステップ、を含む、調製方法を提供する。 |
Author | EGUCHI Hajime SAITO Michiyoshi DAIGO Shinobu YAMAMOTO Kyoko ITOH Tohru HARADA Yui WADA Aya YONEMITSU Yoshikazu KOGUCHI Ryohei |
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DocumentTitleAlternate | PROCÉDÉ DE PRÉPARATION DE POPULATION DE CELLULES ARBORESCENTES 樹状細胞集団の調製方法 |
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Title | METHOD FOR PREPARING DENDRITIC CELL POPULATION |
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