MULTIPLEX PCR PRIMER AND APPLICATION THEREOF

• Main Authors: LIU, Lichun, GE, Liangjin, LIN, Qunting, HUANG, Liang, LIU, Song, LI, Gailing, ZENG, Lidong, HUANG, Shasha
• Format: Patent
• Language: Chinese; English; French
• Published: 23.02.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Provided are multiplex PCR primers, comprising a forward primer and a reverse primer. The forward primer consists of a sequence in one-to-one correspondence with a nucleotide sequence represented by SEQ ID NO:1-SEQ ID NO:25. The sequence in the forward primer has 0-3 nucleotides greater or fewer than the corresponding sequence in SEQ ID NO:1-SEQ ID NO:25. The reverse primer consists of a sequence in one-to-one correspondence with a nucleotide sequence represented by SEQ ID NO:26-SEQ ID NO:38. The sequence in the reverse primer has 0-3 nucleotides greater or fewer than the corresponding sequence in SEQ ID NO:26-SEQ ID NO:38. L'invention concerne des amorces de PCR multiplex, comprenant une amorce sens et une amorce antisens. L'amorce sens est constituée d'une séquence en correspondance un-à-un avec une séquence nucléotidique représentée par SEQ ID NO : 1 à SEQ ID NO : 25. La séquence dans l'amorce sens possède de 0 à 3 nucléotides de plus ou de moins que la séquence correspondante dans SEQ ID NO : 1 à SEQ ID N