PROCESS FOR PRODUCING ACYL AMINO ACIDS EMPLOYING LIPASES
There is provided a microbial cell for producing at least one acyl amino acid, wherein the cell is genetically modified to comprise: - a first genetic mutation that increases the expression relative to a wild type cell of at least one lipase (EC 3.1.1 ) (E1) capable of hydrolysing at least one glyce...
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Format | Patent |
Language | English French |
Published |
10.11.2016
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Abstract | There is provided a microbial cell for producing at least one acyl amino acid, wherein the cell is genetically modified to comprise: - a first genetic mutation that increases the expression relative to a wild type cell of at least one lipase (EC 3.1.1 ) (E1) capable of hydrolysing at least one glyceride to at least one fatty acid wherein the glyceride is a triglyceride; and - a second and a third genetic mutation that increases the expression relative to a wild type cell of: (i) an amino acid-N-acyl-transferase (EC 2.3.1 ) (E2), and (ii) an acyl-CoA synthetase (EC 6.2.1.3) (E3) respectively that enables the cell to convert the fatty acid to at least one acyl amino acid and wherein the cell has a reduced fatty acid degradation capacity.
L'invention concerne une cellule microbienne pour la production d'au moins un acide aminé acylé, la cellule étant génétiquement modifiée pour comprendre : -une première mutation génétique qui augmente l'expression par rapport à une cellule de type sauvage d'au moins une lipase (EC 3.1.1) (E1) capable d'hydrolyser au moins un glycéride en au moins un acide gras, le glycéride étant un triglycéride ; et - une deuxième et une troisième mutation génétique qui augmentent l'expression par rapport à une cellule de type sauvage de : (i) un acide aminé-N-acyl-transférase (EC 2.3.1) (E2), et (ii) une acyl-CoA synthétase (EC 6.2.1.3) (E3) respectivement qui permet à la cellule de convertir l'acide gras en au moins un acide aminé acylé, la cellule ayant une capacité de dégradation des acides gras réduite. |
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AbstractList | There is provided a microbial cell for producing at least one acyl amino acid, wherein the cell is genetically modified to comprise: - a first genetic mutation that increases the expression relative to a wild type cell of at least one lipase (EC 3.1.1 ) (E1) capable of hydrolysing at least one glyceride to at least one fatty acid wherein the glyceride is a triglyceride; and - a second and a third genetic mutation that increases the expression relative to a wild type cell of: (i) an amino acid-N-acyl-transferase (EC 2.3.1 ) (E2), and (ii) an acyl-CoA synthetase (EC 6.2.1.3) (E3) respectively that enables the cell to convert the fatty acid to at least one acyl amino acid and wherein the cell has a reduced fatty acid degradation capacity.
L'invention concerne une cellule microbienne pour la production d'au moins un acide aminé acylé, la cellule étant génétiquement modifiée pour comprendre : -une première mutation génétique qui augmente l'expression par rapport à une cellule de type sauvage d'au moins une lipase (EC 3.1.1) (E1) capable d'hydrolyser au moins un glycéride en au moins un acide gras, le glycéride étant un triglycéride ; et - une deuxième et une troisième mutation génétique qui augmentent l'expression par rapport à une cellule de type sauvage de : (i) un acide aminé-N-acyl-transférase (EC 2.3.1) (E2), et (ii) une acyl-CoA synthétase (EC 6.2.1.3) (E3) respectivement qui permet à la cellule de convertir l'acide gras en au moins un acide aminé acylé, la cellule ayant une capacité de dégradation des acides gras réduite. |
Author | DECKER, Nicole HENNEMANN, Hans-Georg REINECKE, Liv GRAMMANN, Katrin KIRCHNER, Nicole |
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DocumentTitleAlternate | PROCÉDÉ POUR LA PRODUCTION D'ACIDES AMINÉS ACYLÉS À L'AIDE DE LIPASES |
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RelatedCompanies | EVONIK DEGUSSA GMBH DECKER, Nicole HENNEMANN, Hans-Georg REINECKE, Liv GRAMMANN, Katrin KIRCHNER, Nicole |
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SubjectTerms | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
Title | PROCESS FOR PRODUCING ACYL AMINO ACIDS EMPLOYING LIPASES |
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