NOVEL CELL WALL DECONSTRUCTION ENZYMES OF THERMOASCUS AURANTIACUS, MYCELIOPHTHORA FERGUSII (CORYNASCUS THERMOPHILUS), AND PSEUDOCERCOSPORELLA HERPOTRICHOIDES, AND USES THEREOF

• Main Authors: TSANG, ADRIAN, POWLOWSKI, JUSTIN, BUTLER, GREGORY
• Format: Patent
• Language: English; French
• Published: 24.04.2014
• Subjects: APPARATUS THEREFOR; BAKERY PRODUCTS; BAKING; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DYEING LEATHER, FURS, OR SOLID MACROMOLECULAR SUBSTANCES INANY FORM; DYEING OR PRINTING TEXTILES; EDIBLE DOUGHS; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR; FODDER; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; HUMAN NECESSITIES; IMPREGNATING OR COATING OF PAPER; LAUNDERING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PAPER NOT OTHERWISE PROVIDED FOR; PAPER-MAKING; PEPTIDES; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PRESERVATION THEREOF; PRODUCTION OF CELLULOSE; PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCESFROM CELLULOSE-CONTAINING MATERIALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; PULP COMPOSITIONS; REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGYGENERATION, TRANSMISSION OR DISTRIBUTION; REGENERATION OF PULPING LIQUORS; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; TEXTILES; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; TREATMENT OF TEXTILES OR THE LIKE; TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BYADDITION OF MATERIALS; TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS,FABRICS, FEATHERS, OR FIBROUS GOODS MADE FROM SUCH MATERIALS; VINEGAR; WINE

The present invention relates to novel polypeptides and enzymes (e.g., thermostable proteins and enzymes) having activities relating to biomass processing and/or degradation (e.g., cell wall deconstruction), as well as polynucleotides, vectors, cells, compositions and tools relating to same, or functional variants thereof. More particularly, the present invention relates to secreted enzymes that may be isolated from the fungi, Thermoascus aurantiacus strain CBS 181.67, Myceliophthora fergusii (Corynascus thermophilus) strain CBS 405.69, and Pseudocercosporella herpotrichoides strain 494.80. Uses thereof in various industrial processes such as in biofuels, food preparation, animal feed, pulp and paper, textiles, detergents, waste treatment and others are also disclosed. La présente invention concerne de nouveaux polypeptides et de nouvelles enzymes (par exemple des protéines et des enzymes thermostables) ayant des activités associées au traitement et/ou à la dégradation de la biomasse (par exemple la déconstruction de la paroi cellulaire), ainsi que des polynucléotides, des vecteurs, des cellules, des compositions et des outils associés à ceux-ci, ou des variants fonctionnels de ceux-ci. Plus particulièrement, la présente invention concerne des enzymes secrétées qui peuvent être isolées des champignons, de la souche CBS 181.67 de Thermoascus aurantiacus, de la souche CBS 405.69 de Myceliophthora fergusii (Corynascus thermophilus) et de la souche 494.80 de Pseudocercosporella herpotrichoides. L'invention concerne également leurs utilisations dans divers procédés industriels, tels que les biocarburants, la préparation d'aliment, les aliments pour animaux, la pâte et le papier, les textiles, les détergents, le traitement des déchets et d'autres procédés.