GLUCOSE DEHYDROGENASE

Provided are a glucose dehydrogenase originating in Botryotinia fuckeliana, a method for producing the same, and an assay method, a reagent composition and a biosensor each using the aforesaid enzyme. A biosensor for assaying glucose without being affected by dissolved oxygen, characterized by using...

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Main Authors TAKENAKA, RYO, TAKI, SAYAKA, HONDA, MICHINARI
Format Patent
LanguageEnglish
French
Japanese
Published 11.04.2013
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Abstract Provided are a glucose dehydrogenase originating in Botryotinia fuckeliana, a method for producing the same, and an assay method, a reagent composition and a biosensor each using the aforesaid enzyme. A biosensor for assaying glucose without being affected by dissolved oxygen, characterized by using a flavin-bound glucose dehydrogenase that comprises glycopolypeptide (e) or (f): (e) a glycopolypeptide comprising an amino acid sequence represented by SEQ ID NO:5; and (f) a glycopolypeptide comprising an amino acid sequence that is derived from the amino acid sequence represented by SEQ ID NO:5 by substitution, deletion or addition of one to several amino acids and having glucose dehydrogenase activity. L'invention concerne une glucose déshydrogénase provenant de Botryotinia fuckeliana, un procédé de production de celle-ci, et une méthode de dosage, une composition de réactif et un biocapteur, utilisant chacun l'enzyme mentionnée ci-dessus. L'invention concerne un biocapteur pour le dosage du glucose sans être affecté par l'oxygène dissous, caractérisé en ce qu'il utilise une glucose déshydrogénase liée à une flavine qui comprend un glycopolypeptide (e) ou (f) : (e) un glycopolypeptide comprenant une séquence d'acides aminés représentée par SEQ ID No:5 ; et (f) un glycopolypeptide comprenant une séquence d'acides aminés qui est issue de la séquence d'acides aminés représentée par SEQ ID No:5 par substitution, délétion ou addition d'un à plusieurs acides aminés et ayant une activité glucose déshydrogénase.
AbstractList Provided are a glucose dehydrogenase originating in Botryotinia fuckeliana, a method for producing the same, and an assay method, a reagent composition and a biosensor each using the aforesaid enzyme. A biosensor for assaying glucose without being affected by dissolved oxygen, characterized by using a flavin-bound glucose dehydrogenase that comprises glycopolypeptide (e) or (f): (e) a glycopolypeptide comprising an amino acid sequence represented by SEQ ID NO:5; and (f) a glycopolypeptide comprising an amino acid sequence that is derived from the amino acid sequence represented by SEQ ID NO:5 by substitution, deletion or addition of one to several amino acids and having glucose dehydrogenase activity. L'invention concerne une glucose déshydrogénase provenant de Botryotinia fuckeliana, un procédé de production de celle-ci, et une méthode de dosage, une composition de réactif et un biocapteur, utilisant chacun l'enzyme mentionnée ci-dessus. L'invention concerne un biocapteur pour le dosage du glucose sans être affecté par l'oxygène dissous, caractérisé en ce qu'il utilise une glucose déshydrogénase liée à une flavine qui comprend un glycopolypeptide (e) ou (f) : (e) un glycopolypeptide comprenant une séquence d'acides aminés représentée par SEQ ID No:5 ; et (f) un glycopolypeptide comprenant une séquence d'acides aminés qui est issue de la séquence d'acides aminés représentée par SEQ ID No:5 par substitution, délétion ou addition d'un à plusieurs acides aminés et ayant une activité glucose déshydrogénase.
Author TAKENAKA, RYO
TAKI, SAYAKA
HONDA, MICHINARI
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Snippet Provided are a glucose dehydrogenase originating in Botryotinia fuckeliana, a method for producing the same, and an assay method, a reagent composition and a...
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SubjectTerms APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
Title GLUCOSE DEHYDROGENASE
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