METHOD FOR COLLECTION OF NUCLEIC ACID DERIVED FROM MAMMALIAN CELL, METHOD FOR ANALYSIS OF NUCLEIC ACID, AND KIT FOR COLLECTION OF FECES
Disclosed is a method for collecting a nucleic acid derived from a mammalian cell from feces, which is so convenient that the method can be applied to feces collected in routine health checkups or the like and enables the preferential collection of the nucleic acid over a nucleic acid derived from a...
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Format | Patent |
Language | English French Japanese |
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25.11.2010
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Abstract | Disclosed is a method for collecting a nucleic acid derived from a mammalian cell from feces, which is so convenient that the method can be applied to feces collected in routine health checkups or the like and enables the preferential collection of the nucleic acid over a nucleic acid derived from an indigenous bacterium colonized in the intestinal tract. The method for collecting a nucleic acid derived from a mammalian cell is characterized by comprising the steps of: (A) adding the feces to a treatment solution having a high salt concentration to prepare a feces sample and immersing the feces in the treatment solution in the feces sample for a predetermined period; (B), subsequent to step (A), collecting a solid component from the feces sample; and (C) collecting the nucleic acid from the solid component that has been collected in step (B).
La présente invention concerne un procédé de collecte d'un acide nucléique dérivé d'une cellule de mammifère provenant de fèces, ledit procédé étant pratique au point de pouvoir être appliqué aux fèces collectées lors des bilans de santé habituels ou analogues et de permettre la collecte préférentielle de l'acide nucléique sur un acide nucléique dérivé d'une bactérie indigène colonisant le tractus intestinal. Ledit procédé de collecte d'un acide nucléique dérivé d'une cellule de mammifère est caractérisé en ce qu'il comprend les étapes consistant à : (A) ajouter les fèces à une solution de traitement présentant une concentration élevée en sels pour préparer un échantillon de fèces et laisser les fèces immergées dans la solution de traitement de l'échantillon de fèces pendant un laps de temps prédéfini ; (B) après l'étape (A), collecter un composant solide à partir de l'échantillon de fèces ; et (C) collecter l'acide nucléique à partir du composant solide qui a été collecté à l'étape (B). |
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AbstractList | Disclosed is a method for collecting a nucleic acid derived from a mammalian cell from feces, which is so convenient that the method can be applied to feces collected in routine health checkups or the like and enables the preferential collection of the nucleic acid over a nucleic acid derived from an indigenous bacterium colonized in the intestinal tract. The method for collecting a nucleic acid derived from a mammalian cell is characterized by comprising the steps of: (A) adding the feces to a treatment solution having a high salt concentration to prepare a feces sample and immersing the feces in the treatment solution in the feces sample for a predetermined period; (B), subsequent to step (A), collecting a solid component from the feces sample; and (C) collecting the nucleic acid from the solid component that has been collected in step (B).
La présente invention concerne un procédé de collecte d'un acide nucléique dérivé d'une cellule de mammifère provenant de fèces, ledit procédé étant pratique au point de pouvoir être appliqué aux fèces collectées lors des bilans de santé habituels ou analogues et de permettre la collecte préférentielle de l'acide nucléique sur un acide nucléique dérivé d'une bactérie indigène colonisant le tractus intestinal. Ledit procédé de collecte d'un acide nucléique dérivé d'une cellule de mammifère est caractérisé en ce qu'il comprend les étapes consistant à : (A) ajouter les fèces à une solution de traitement présentant une concentration élevée en sels pour préparer un échantillon de fèces et laisser les fèces immergées dans la solution de traitement de l'échantillon de fèces pendant un laps de temps prédéfini ; (B) après l'étape (A), collecter un composant solide à partir de l'échantillon de fèces ; et (C) collecter l'acide nucléique à partir du composant solide qui a été collecté à l'étape (B). |
Author | TANIGAMI, YASUO NAGAOKA, TOMONORI ASAKURA, MASANORI |
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DocumentTitleAlternate | PROCÉDÉ DE COLLECTE D'ACIDE NUCLÉIQUE DÉRIVÉ DE CELLULE DE MAMMIFÈRE, PROCÉDÉ D'ANALYSE D'ACIDE NUCLÉIQUE, ET NÉCESSAIRE DE COLLECTE DE FÈCES |
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RelatedCompanies | TANIGAMI, YASUO NAGAOKA, TOMONORI ASAKURA, MASANORI OLYMPUS CORPORATION |
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Snippet | Disclosed is a method for collecting a nucleic acid derived from a mammalian cell from feces, which is so convenient that the method can be applied to feces... |
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SubjectTerms | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE VINEGAR WINE |
Title | METHOD FOR COLLECTION OF NUCLEIC ACID DERIVED FROM MAMMALIAN CELL, METHOD FOR ANALYSIS OF NUCLEIC ACID, AND KIT FOR COLLECTION OF FECES |
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