CANCER-ASSOCIATED ANTIGEN ANALOG PEPTIDE AND UTILIZATION OF THE SAME

• Main Authors: MAEDA, HIROO, OKUBO, MITSUO, TAKEDA, SATORU
• Format: Patent
• Language: English; French; Japanese
• Published: 12.10.2006
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

By using an experimental system for detecting a combination of DC4 with IL4 or IFN?, it is confirmed that ovarian cancer antigen-specific T cells occur in the peripheral blood of an ovarian cancer patient. By using an analog peptide of the core protein MUC16 of an ovarian cancer-associated antibody C125, it is then clarified that antigen-specific CD4-positive T cells occur at an average frequency of about 4% in the peripheral monocytes of patients and normal subjects. By analyzing the T cell epitope in the core protein MUC16 of the ovarian cancer-associated antigen CA125, the amino acid sequence of a shorter epitope FTLNFTITN (SEQ ID NO:1) is determined. Moreover, it is found out that an analog peptide OVCA 11:GHTAPGPLLVPFTLNFTITN (SEQ ID NO:11) is suitable for activating T cells. En utilisant un système expérimental pour la détection d'une combinaison de DC4 avec IL4 ou IFN?, on confirme que les cellules T spécifiques de l'antigène du cancer de l'ovaire apparaissent dans le sang périphérique d'un patient atteint d'un cancer de l'ovaire. Au moyen de l'utilisation d'un peptide analogue de l'apoenzyme MUC16 d'un anticorps C125 associé au cancer de l'ovaire, il est alors clarifié que les cellules T CD4-positives spécifiques de l'antigène apparaissent à une fréquence moyenne de 4% environ dans les monocytes périphériques des patients et des sujets normaux. En analysant le déterminant antigénique de la cellule T dans l'apoenzyme MUC16 de l'antigène CA125 associé au cancer de l'ovaire, la séquence d'acides aminés d'un déterminant antigénique plus court FTLNFTITN (SEQ ID NO:1) est déterminée. En outre, on découvre qu'un peptide analogue OVCA 11:GHTAPGPLLVPFTLNFTITN (SEQ ID NO:11) est adéquat pour l'activation des cellules T.