METHODS OF PROTEIN PURIFICATION AND RECOVERY

• Main Authors: BABUKA, SUSAN, WOLFE, SID, SHIRLEY, BRET, A, ESIKOVA, IRINA, FORDHAM, DENNIS
• Format: Patent
• Language: English; French
• Published: 02.05.2002
• Edition: 7
• Subjects: CHEMISTRY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

Improved methods for purification and recovery of interferon-beta (IFN- beta ) and compositions comprising substantially monomeric IFN- beta are provided. In one purification method, substantially purified IFN- beta or variant thereof is precipitated and then dissolved in a guanidine hydrochloride (...