SELF-COMPLEMENTARY MOLECULAR PROBE AND METHODS USING IT

• Main Authors: RHOADS, JAMES, A, GENTILE-DAVEY, MARIA, C, CHAN, CLIFFORD, M, ELMORE, KRISTIN
• Format: Patent
• Language: English; French
• Published: 28.08.2003
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Disclosed herein is a molecular probe comprising a 3' portion; a 5' portion; and a center portion, that is complementary to a target sequence between the 3' and 5' portions. The 3' portion is complementary to the 5' portion such that when the 3' portion is hydridized to the 5' portion the probe has a stem loop structure. Additionally, parts of either or both of the 3' or 5' portions of the probe are complementary to the target sequence. Methods for using such probes are also provided. La présente invention concerne une sonde moléculaire comprenant un segment 3', un segment 5', et un segment central qui est complémentaire d'une séquence cible comprise entre les segments 3' et 5'. Le segment 3' est complémentaire du segment 5' de sorte que lorsque le segment 3' est hybridé avec le segment 5', la sonde a une structure tige et boucle. De plus, des parties de l'un et/ou l'autre des segments 3' ou 5' de l'échantillon sont complémentaires à la séquence cible. Cette invention concerne également des procédés permettant l'utilisation de sondes de ce type.