NOVEL PROMOTER OF GENE EXPRESSION
The invention provides an isolated DNA molecule comprising a promoter sequence, said promoter sequence being a Pm or a Pu promoter of a TOL plasmid, said promoter sequence having sequence modifications in its 10 region together with DNA constructs, expression vectors and transformed cells containing...
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Main Authors | , |
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Format | Patent |
Language | English French |
Published |
16.11.2000
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Edition | 7 |
Subjects | |
Online Access | Get full text |
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Abstract | The invention provides an isolated DNA molecule comprising a promoter sequence, said promoter sequence being a Pm or a Pu promoter of a TOL plasmid, said promoter sequence having sequence modifications in its 10 region together with DNA constructs, expression vectors and transformed cells containing such molecules. Also provided by the invention is a method for assaying promoter activity, comprising expressing in an antibiotic-susceptible (i.e. sensitive) host cell, an antibiotic resistance gene under the control of the promoter to be assayed, and assessing the growth of said cell in the presence of said antibiotic.
La présente invention concerne une molécule d'ADN isolée contenant une séquence promoteur, cette séquence promoteur étant un promoteur Pm ou Pu d'un plasmide TOL et présentant des modifications de séquence dans sa région 10; une structure d'ADN, des vecteurs d'expression et des cellules transformées contenant de telles molécules. On décrit également un procédé permettant d'estimer l'activité du promoteur, ce procédé consistant à exprimer dans une cellule sensible à un antibiotique (c'est-à-dire réactive), un gène de résistance à l'antibiotique sous le contrôle du promoteur devant être estimé puis à évaluer la croissance de ladite cellule en présence dudit antibiotique. |
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AbstractList | The invention provides an isolated DNA molecule comprising a promoter sequence, said promoter sequence being a Pm or a Pu promoter of a TOL plasmid, said promoter sequence having sequence modifications in its 10 region together with DNA constructs, expression vectors and transformed cells containing such molecules. Also provided by the invention is a method for assaying promoter activity, comprising expressing in an antibiotic-susceptible (i.e. sensitive) host cell, an antibiotic resistance gene under the control of the promoter to be assayed, and assessing the growth of said cell in the presence of said antibiotic.
La présente invention concerne une molécule d'ADN isolée contenant une séquence promoteur, cette séquence promoteur étant un promoteur Pm ou Pu d'un plasmide TOL et présentant des modifications de séquence dans sa région 10; une structure d'ADN, des vecteurs d'expression et des cellules transformées contenant de telles molécules. On décrit également un procédé permettant d'estimer l'activité du promoteur, ce procédé consistant à exprimer dans une cellule sensible à un antibiotique (c'est-à-dire réactive), un gène de résistance à l'antibiotique sous le contrôle du promoteur devant être estimé puis à évaluer la croissance de ladite cellule en présence dudit antibiotique. |
Author | VALLA, SVEIN WINTHER-LARSEN, HANNE |
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DocumentTitleAlternate | NOUVEAU PROMOTEUR DE L'EXPRESSION GENIQUE |
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RelatedCompanies | DZIEGLEWSKA, HANNA, EVA LEIV EIRIKSSON NYFOTEK AS VALLA, SVEIN WINTHER-LARSEN, HANNE |
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Snippet | The invention provides an isolated DNA molecule comprising a promoter sequence, said promoter sequence being a Pm or a Pu promoter of a TOL plasmid, said... |
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SubjectTerms | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
Title | NOVEL PROMOTER OF GENE EXPRESSION |
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