Mutant xylanase, manufacturing method and use therefor, and method for manufacturing saccharified lignocellulose

• Main Authors: Tamai Hiroki, Okakura Kaoru, Inoue Atsushi, Osabe Masami, Yanai Hisaaki, Yokoyama Fumikazu
• Format: Patent
• Language: English
• Published: 14.02.2017
• Subjects: ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES; BAKERY PRODUCTS; BAKING; BEER; BIOCHEMISTRY; BLEACHING LEATHER OR FURS; CANDLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; DETERGENT COMPOSITIONS; DETERGENTS; DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS,YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS; EDIBLE DOUGHS; ENZYMOLOGY; FATTY ACIDS THEREFROM; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR; HUMAN NECESSITIES; LAUNDERING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PRESERVATION THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECOVERY OF GLYCEROL; RESIN SOAPS; SACCHARIDES, OTHER THAN SUCROSE, OBTAINED FROM NATURAL SOURCESOR BY HYDROLYSIS OF NATURALLY OCCURRING DI-, OLIGO- ORPOLYSACCHARIDES; SOAP OR SOAP-MAKING; SPIRITS; SUGAR INDUSTRY; SUGARS; TEXTILES; TREATMENT OF TEXTILES OR THE LIKE; TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BYADDITION OF MATERIALS; USE OF SINGLE SUBSTANCES AS DETERGENTS; VINEGAR; WINE

What is aimed at is provision of an inexpensive and efficient saccharification method for lignocellulose using a thermostable xylanase and provision of a mutant xylanase that has a substitute amino acid residue, and that exhibits stable activity even under severe conditions in which enzymes easily inactivate, and that provides an initial rate of reaction not significantly reduced as compared to a wild-type xylanase corresponding to the mutant xylanase. Provided is a method of producing a saccharified product of lignocellulose, including contacting a lignocellulosic raw material with a thermostable xylanase, and a mutant xylanase that provides an initial rate of reaction that is at least 70% of that provided by a wild-type xylanase corresponding thereto, that has a xylanase activity after heat treatment at 50° C. for 24 hours that is at least 50% of its xylanase activity before the heat treatment, and that has a substitute amino acid residue.