Immunometric assay kit and method applicable to whole cells

• Main Author: CARRIERE DOMINIQUE
• Format: Patent
• Language: English
• Published: 08.10.2002
• Edition: 7
• Subjects: GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TECHNICAL SUBJECTS COVERED BY FORMER USPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS; TESTING

A process and kit which permit quantitative measurement in a single analysis step of a target antigen of a whole cell. The process and kit utilize a solid support onto which is bound at least one antibody that binds to and immobilizes the subject whole cell at a surface antigenic site other than the target antigen. A second, labeled antibody specific for the target antigen is then added, and the binding is then quantitatively measured by analytical means. The process of this invention can provide rapid quantitative assay, e.g., less than one hour, of multiple samples.