In vitro phage lambda DNA packaging system

A bacterial preparation capable of packaging phage lambda DNA is disclosed. This preparation is in lyophilized form and is stable at ambient temperature. In a preferred form, the preparation contains an over-expressed terminase protein, is prepared from the bacterial strain E. coli Cla [ lambda c/85...

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Main Authors FRACKMAN; SUSAN G, JOLLY; JAMES F, FRANCISKOVICH; PHILLIP P, LUHM; ROBERT A, RIEDL; WILLIAM A
Format Patent
LanguageEnglish
Published 16.04.1996
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Abstract A bacterial preparation capable of packaging phage lambda DNA is disclosed. This preparation is in lyophilized form and is stable at ambient temperature. In a preferred form, the preparation contains an over-expressed terminase protein, is prepared from the bacterial strain E. coli Cla [ lambda c/857 Sam7 DELTA (cos-Nu1-A)::Knr]/ lambda pTER and is capable of a packaging efficiency of at least 1x108 pfu/ mu g wild type lambda DNA. The present invention is also a method of creating a phage lambda DNA packaging extract comprising the steps of preparing a bacterial extract capable of packaging phage lambda and lyophilizing said extract.
AbstractList A bacterial preparation capable of packaging phage lambda DNA is disclosed. This preparation is in lyophilized form and is stable at ambient temperature. In a preferred form, the preparation contains an over-expressed terminase protein, is prepared from the bacterial strain E. coli Cla [ lambda c/857 Sam7 DELTA (cos-Nu1-A)::Knr]/ lambda pTER and is capable of a packaging efficiency of at least 1x108 pfu/ mu g wild type lambda DNA. The present invention is also a method of creating a phage lambda DNA packaging extract comprising the steps of preparing a bacterial extract capable of packaging phage lambda and lyophilizing said extract.
Author LUHM; ROBERT A
JOLLY; JAMES F
RIEDL; WILLIAM A
FRACKMAN; SUSAN G
FRANCISKOVICH; PHILLIP P
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Snippet A bacterial preparation capable of packaging phage lambda DNA is disclosed. This preparation is in lyophilized form and is stable at ambient temperature. In a...
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SubjectTerms BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Title In vitro phage lambda DNA packaging system
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