Process for the preparation of fermentation broth for coenzyme B12 and other corrinoid production

• Main Authors: SIKE; VALERIA, LANGER; MARIA, SZEMLER; LASZLO, PECHANY; EVA C, HRUBY; GYULA
• Format: Patent
• Language: English
• Published: 21.04.1987
• Edition: 4
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a process for the preparation of a fermentation broth containing coenzyme B12, with a new, anaerobic, mesophilic, methane-producing mixed micropopulation, under anaerobic, septic conditions, using a new broth containing methanol, precursors and partially known nutrients, In the new process according to the invention the improvement consists in (a) removing 5 to 15 volume percent of the inoculum fermentation broth containing the anaerobic, mesophilic, methan-producing new mixed micropopulation and replacing same with an equal volume of a broth containing cornsteep liquor hydrolysate and/or corn slops of hydrolysate thereof and other known nutrients, the number and concentration of which has been reduced, for 6 to 8 days, and if desired, further manufacturing the fermentation broth removed, (b) after reaching an assimilation velocity of at least 0.15 to 0.2 g methanol/lit. fermentation broth/hour, continuing removal of the fermentation broth as described in step (a) but replacing same with a broth supplemented with ammonium sulfate and o-xylidine, and continuing fermentation for 6 to 8 days, and if desired, further manufacturing the fermentation broth removed, (c) after achieving an assimilation velocity of 0.2 to 0.3 g methanol/lit. fermentation broth/hour-i.e. after converting the inoculum fermentation broth into a maintaining fermentation broth-continuing removal of the fermentation broth and addition of fresh broth as described in step (b), and if desired, interrupting the removal of fermentation broth for several days, and (d) adding of the removed maintaining fermentation broth on the first day nutrients according to step (b), and if desired, on the second day only methanol, cornsteep liquor or cornsteep liquor hydrolysate and ammonium carbonate and further manufacturing the fermentation broth obtained, which is suitable for batchwise production, and (e) repeating steps (a), (c) and (d) daily.