Method of measuring creatine kinase activity

• Main Authors: KAWARABUKI; SEIICHI, INAGAWA; MASANOBU, SAI; TOSHIYUKI, MIWA; NAOTO
• Format: Patent
• Language: English
• Published: 05.10.1982
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

A method and composition for use therein for measuring the activity of creatine kinase (hereinafter referred to as "CK") having good storage stability is disclosed. More particularly, a composition for measurement of CK activity which comprises as main components phosphoglycerate kinase (hereinafter referred to a "PGK") which catalyzes the following reaction 2 and glyceraldehyde-3-phosphate dehydrogenase (hereinafter referred to as "GAPDH") which catalyzes the following reaction 3. Reaction 1 is catalyzed by the creatine kinase. Reaction 1 Creatinephosphate+Adenosine-5'-diphosphate (hereinafter referred to as "ADP")-><-Creatine+Adenosine-5'-triphosphate (hereinafter referred to as "ATP") Reaction 2 ATP+3-Phosphoglycerate-><-ADP+1,3-Diphosphoglycerate Reaction 3 1,3-Diphosphoglycerate+Reduce beta -nicotinamideadenine dinucleotide (hereinafter referred to as "NADH")-><-Glyceraldehyde-3-phosphate+ beta -nicotinamideadenine dinucleotide (hereinaf