METHOD FOR PRODUCING HUMAN COLLAGEN STRUCTURES WITH CONTROLLED CHARACTERISTICS

• Main Authors: ROJAS GARCÍA, Octavio Israel, AGUILAR ALEMÁN, Juan Pablo, MIRAMONTES BEAS, Eduardo, CARDOSO HERNÁNDEZ, Grecia Andrea, CAMACHO PÉREZ, Beni, AGUILLÓN ESTRADA, Brenda Karen
• Format: Patent
• Language: English
• Published: 22.02.2024
• Subjects: CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; SEPARATION; TRANSPORTING

A method for producing human collagen structures with controlled characteristics, having the following stages: a) Tissue conditioning b) Pre-treatment; b1) final washings with distilled water; c) extraction by enzymatic hydrolysis by subjecting the tissue to a solution of acetic acid with pepsin; d) precipitation, where the resulting collagen solution is brought to a high concentration by adding sodium chloride and where the fibers recovered from the sieve are solubilized again in an acetic acid solution; e) dynamic dialysis to purify the solution from the excess of salt present in the collagen solution; f) lyophilization at −40° C. and a vacuum pressure of 0.04 mbar (4 Pa); g) molding or second lyophilization, where a concentration is chosen and collagen is solubilized in an acetic acid solution; once solubilized, collagen is placed in molds to generate the desired structure and once again, the solution is lyophilized; h) crosslinking, where the collagen pieces are subjected to a formaldehyde vapor atmosphere in a crosslinking apparatus and i) pressing, where the collagen structure is subjected to a mechanical force of 400-5000 N compacting to a value of 0.01-10 mm and increasing its fibrillar density.