RAPID ASSAY FOR APOL1 G0 PROTEIN

ELISA-type assays, lateral flow test strips, methods, and systems are provided for detecting APOL1 G0 in bodily samples including blood, serum, or plasma to provide a rapid rule-in test for the ApoL1 G0 genotype. The assays exploit the differential affinity of serum resistance-associated protein (SR...

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Main Authors DARBY, Martyn Kerry, AXTMAN, Matthew, LESLIE, Patrick, Vaughan, Emily, SANFORD, Isaac Gilliam
Format Patent
LanguageEnglish
Published 30.11.2023
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Abstract ELISA-type assays, lateral flow test strips, methods, and systems are provided for detecting APOL1 G0 in bodily samples including blood, serum, or plasma to provide a rapid rule-in test for the ApoL1 G0 genotype. The assays exploit the differential affinity of serum resistance-associated protein (SRA) for wild-type APOL1 (designated 'G0') over the other variants G1 and G2 to specifically detect APOL1 G0. Results with human plasma samples (N=130) from all six genotypes (G0/G0, G0/G1, G0/G2, G1/G2, G2/G2, G1/G1) show the assay can detect APOL1 G0 from plasma with 100% concordance with genotyping. The assay fulfills an unmet need for a rapid test (i.e., within about an hour) for determining APOL1 variant status in deceased kidney donors which represent 72% of donated kidneys.
AbstractList ELISA-type assays, lateral flow test strips, methods, and systems are provided for detecting APOL1 G0 in bodily samples including blood, serum, or plasma to provide a rapid rule-in test for the ApoL1 G0 genotype. The assays exploit the differential affinity of serum resistance-associated protein (SRA) for wild-type APOL1 (designated 'G0') over the other variants G1 and G2 to specifically detect APOL1 G0. Results with human plasma samples (N=130) from all six genotypes (G0/G0, G0/G1, G0/G2, G1/G2, G2/G2, G1/G1) show the assay can detect APOL1 G0 from plasma with 100% concordance with genotyping. The assay fulfills an unmet need for a rapid test (i.e., within about an hour) for determining APOL1 variant status in deceased kidney donors which represent 72% of donated kidneys.
Author DARBY, Martyn Kerry
AXTMAN, Matthew
LESLIE, Patrick
Vaughan, Emily
SANFORD, Isaac Gilliam
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MEASURING
PHYSICS
TESTING
Title RAPID ASSAY FOR APOL1 G0 PROTEIN
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