YEAST CELLS HAVING DISRUPTED PATHWAY FROM DIHYDROXYACETONE PHOSPHATE TO GLYCEROL

• Main Authors: Rush, Brian J, Hause, Benjamin Matthew, Roberg-Perez, Kevin, Suominen, Pirrko, Koivuranta, Kari, Aristidou, Aristos, McMullin, Thomas William, Dundon, Catherine Asleson
• Format: Patent
• Language: English
• Published: 13.09.2018
• Subjects: CONSTRUCTION OF ROADS, RAILWAYS, OR BRIDGES; CONVEYING; FIXED CONSTRUCTIONS; HANDLING THIN OR FILAMENTARY MATERIAL; MACHINES FOR MAKING RAILWAYS OF ALL KINDS; PACKING; PERFORMING OPERATIONS; PERMANENT WAY; PERMANENT-WAY TOOLS; PNEUMATIC TUBE CONVEYORS; SHOP CONVEYOR SYSTEMS; STORING; TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING ORTIPPING; TRANSPORTING

Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the genera Kluyveromyces, Candida or Issatchenkia. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.